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PXD001348

PXD001348 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomics analysis of the effect of deletion and mutation of eIF4E binding proteins Caf20 and Eap1 on yeast via LC-MSMS.
DescriptionThere are multiple translational control pathways. These include pathways involving eIF4E binding proteins (4E-BPs), which inhibit translation by binding and sequestering the 5’ cap binding protein eIF4E away from its partner eIF4G. Saccharomyces cerevisiae has two 4E-BPs: Caf20p and Eap1p; and, microarray analysis has shown that each regulates different subsets of mRNAs. To assess the effect of these different regulatory responses at the protein level, we used label-free mass spectrometry to compare the proteomes of wild-type strain with those of caf20Δ and eap1Δ single mutant strains; caf20Δ and eap1Δ double mutant strain; and, caf20Δ mutant strain transformed with one plasmid containing a mutated version of caf20. These analyses indicate that Caf20p and Eap1p may compensate for each other loss, and also reveal that Caf20p participates in translational control pathways that are independent of eIF4E binding.
HostingRepositoryPRIDE
AnnounceDate2016-05-04
AnnouncementXMLSubmission_2016-05-04_08:01:24.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD001348
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterDavid Talavera
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListdeamidated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentDionex instrument model; Mascot; Progenesis LC-MS; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-09-19 07:47:41ID requested
12016-05-04 08:01:25announced
Publication List
Castelli LM, Talavera D, Kershaw CJ, Mohammad-Qureshi SS, Costello JL, Rowe W, Sims PF, Grant CM, Hubbard SJ, Ashe MP, Pavitt GD, The 4E-BP Caf20p Mediates Both eIF4E-Dependent and Independent Repression of Translation. PLoS Genet, 11(5):e1005233(2015) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Yeast, Label-free quantification, LC-MSMS
Contact List
Graham Pavitt
contact affiliationFaculty of Life Science, University of Manchester, United Kingdom
contact emailgraham.pavitt@manchester.ac.uk
lab head
David Talavera
contact affiliationUniversity of Manchester
contact emailDavid.Talavera@manchester.ac.uk
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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