PXD014976 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Arctigenin attenuates diabetic kidney disease through the activation of PP2A in podocytes |
Description | Arctigenin (ATG) is a major component of Fructus Arctii, which as a traditional herbal remedy reduced proteinuria in diabetic patients. However, whether ATG specifically provided renoprotection in DKD was not known. Here we report that ATG administration is sufficient to attenuate proteinuria and podocyte injury in mouse models of diabetes. Transcriptomic analysis of diabetic mouse glomeruli showed that cell adhesion and inflammation are two key pathways affected by ATG treatment, and mass spectrometry analysis identified protein phosphatase 2A (PP2A) as one of the top ATG-interacting proteins in renal cells. Enhanced PP2A activity by ATG reduces p65 NF-κB-mediated inflammatory response and high glucose-induced migration in cultured podocytes via its interaction with Drebrin-1. Importantly, podocyte-specific Pp2a deletion in mice exacerbates DKD injury and abrogates the ATG-mediated renoprotection. Collectively, our results clearly demonstrate a renoprotective mechanism of ATG via PP2A activation and establish PP2A as a potential target for DKD progression. |
HostingRepository | PRIDE |
AnnounceDate | 2019-11-08 |
AnnouncementXML | Submission_2019-11-08_09:07:05.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD014976 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Tong Liu |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | Phospho; Oxidation; Acetyl; Carbamidomethyl |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-08-12 00:57:35 | ID requested | |
⏵ 1 | 2019-11-08 09:07:06 | announced | |
Publication List
Zhong Y, Lee K, Deng Y, Ma Y, Chen Y, Li X, Wei C, Yang S, Wang T, Wong NJ, Muwonge AN, Azeloglu EU, Zhang W, Das B, He JC, Liu R, Arctigenin attenuates diabetic kidney disease through the activation of PP2A in podocytes. Nat Commun, 10(1):4523(2019) [pubmed] |
Keyword List
submitter keyword: Diabetic kidney disease, Arctigenin, podocytes, PP2A, actin cytoskeleton, Drebrin-1 |
Contact List
John Cijiang He |
contact affiliation | John Cijiang He, MD/PhD Division of Nephrology, Box 1243 Mount Sinai School of Medicine One Gustave L Levy Place, New York NY 10029 Tel: 212-659-1703, Fax: 212-987-0389 |
contact email | cijiang.he@mssm.edu |
lab head | |
Tong Liu |
contact affiliation | Rutgers University |
contact email | linto@njms.rutgers.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD014976 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014976
- Label: PRIDE project
- Name: Arctigenin attenuates diabetic kidney disease through the activation of PP2A in podocytes