Aspergillus niger is an opportunistic pathogen commonly found in a variety of indoor and out-door environments. Environmental isolates of A. niger taken from a pig farm were resistant to itraconazole and in-depth investigations were conducted to better understand cellular re-sponses during growth when exposed to an antifungal. Using a combination of cultivation techniques, antibiotic stress-testing, and label-free pro-teomics, this study has investigated the physiological and metabolic responses of A. niger to differing levels of antifungal stress.