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<DatasetSummary announceDate="2025-01-04" hostingRepository="PRIDE" title="Feed-forward stimulation of CAMK2 by the oncogenic pseudokinase PEAK1 generates an ‘actionable’ signalling axis in triple negative breast cancer">
<Description>To characterize the interactomes of PEAK1 and PEAK2 homodimers, and the PEAK1/PEAK2 heterodimer, we applied bimolecular complementation affinity purification coupled with tandem mass spectrometry (BiCAP-MS/MS), a recently-developed technique that specifically identifies the interactors of any pair of interacting proteins while excluding those binding to individual components. Appropriate pairs of V1- and V2-tagged proteins were co-transfected into HEK293T cells, and then specific dimeric complexes were affinity purified with GFP-Trap nanobody and associated proteins identified by LC-MS/MS. Proteins exhibiting significantly increased abundance in particular PEAK dimers versus the Venus control were identified by label-free quantitative MS analysis, and are presented in volcano plots.</Description>
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<cvParam cvRef="MS" accession="MS:1000586" name="contact name" value="Xue Yang"/>
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<cvParam cvRef="MS" accession="MS:1000590" name="contact affiliation" value="Cancer Program, Monash Biomedicine Discovery Institute, Clayton, VIC 3800, Australia.
Department of Biochemistry and Molecular Biology, Monash University, Melbourne, VIC 3800, Australia."/>
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<cvParam cvRef="MS" accession="MS:1000586" name="contact name" value="Roger John Daly"/>
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<cvParam cvRef="MS" accession="MS:1000590" name="contact affiliation" value="Cancer Program, Monash Biomedicine Discovery Institute, Clayton, VIC 3800, Australia. Department of Biochemistry and Molecular Biology, Monash University, Melbourne, VIC 3800, Australia."/>
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<cvParam cvRef="MS" accession="MS:1002866" name="Reference" value="Yang X, Ma X, Zhao T, Croucher DR, Nguyen EV, Clark KC, Hu C, Latham SL, Bayly-Jones C, Nguyen VCB, Budnar S, Shin S, Nguyen LK, Cotton TR, Chueh AC, Sian TCLK, Stratton MM, Ellisdon AM, Daly RJ. Feed-forward stimulation of CAMK2 by the oncogenic pseudokinase PEAK1 generates a therapeutically "actionable" signalling axis in triple negative breast cancer. bioRxiv. 2024:2024.02.14.580406"/>
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