PXD000107

PXD000107 is an original dataset announced via ProteomeXchange.

Title	Pseudomonas aeruginosa inner membrane proteome
Description	The present dataset provides the first description of Pseudomonas aeruginosa inner membrane proteome. To achieve this, we combined efficient separation of membranes from PAO1 reference strain using discontinuous sucrose gradient centrifugation and mass spectrometry-based proteomic analysis. Three biological replicates were prepared and analysed twice by nanoLC-MS/MS (LTQ-Orbitrap Velos pro). Using Mascot (v.2.4), IRMa (v.1.30.4) and homemade hEIDI (v.1.13), a core list of 991 non-redundant proteins was established.
HostingRepository	PRIDE
AnnounceDate	2013-07-04
AnnouncementXML	Submission_2013-07-04_06:47:00.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000107
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yohann Couté
SpeciesList	scientific name: Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228); NCBI TaxID: 208964;
ModificationList	No applicable mass modifications
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2012-12-13 07:06:09	ID requested
1	2013-07-04 06:26:53	announced
1	2014-07-28 00:27:18	announced
⏵ 2	2013-07-04 06:47:00	announced	Add reference

Casabona MG, Vandenbrouck Y, Attree I, Cout, é Y, Proteomic characterization of Pseudomonas aeruginosa PAO1 inner membrane. Proteomics, 13(16):2419-23(2013) [pubmed]

submitter keyword: LC-MS/MS

Yohann Couté
contact affiliation	Laboratoire d'Etude de la Dynamique des Protéomes
contact email	yohann.coute@cea.fr

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• PRIDE
  1. 28014
     1. InstrumentRef: INSTRUMENT_1
     2. Label: Pa IM
     3. ModificationList: No applicable mass modifications,
     4. Name: Identification of P. aeruginosa inner membrane proteins by mass spectrometry-based proteomics
     5. PublicationRef: PMID23744604