PXD017935

PXD017935 is an original dataset announced via ProteomeXchange.

Title	Methylation of Salmonella Typhimurium flagella promotes bacterial adhesion and host cell invasion
Description	The long external filament of bacterial flagella is composed of several thousand copies of a single protein, flagellin. Here, we explore the role played by lysine methylation of flagellin in Salmonella, which requires the methylase FliB. We show that both flagellins of Salmonella enterica serovar Typhimurium, FliC and FljB, are methylated at surface-exposed lysine residues by FliB. A Salmonella Typhimurium mutant deficient in flagellin methylation is outcompeted for gut colonization in a gastroenteritis mouse model, and methylation of flagellin promotes bacterial invasion of epithelial cells in vitro. Lysine methylation increases the surface hydrophobicity of flagellin and enhances flagella-dependent adhesion of Salmonella to phosphatidylcholine vesicles and epithelial cells.
HostingRepository	PRIDE
AnnounceDate	2020-04-27
AnnouncementXML	Submission_2020-04-26_23:22:29.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD017935
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christoph Krisp
SpeciesList	scientific name: Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344; NCBI TaxID: 216597;
ModificationList	Methyl; Dimethyl; Oxidation; Carbamidomethyl; Trimethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-09 03:38:18	ID requested
⏵ 1	2020-04-26 23:22:31	announced

Horstmann JA, Lunelli M, Cazzola H, Heidemann J, K, ü, hne C, Steffen P, Szefs S, Rossi C, Lokareddy RK, Wang C, Lemaire L, Hughes KT, Uetrecht C, Schl, ü, ter H, Grassl GA, Stradal TEB, Rossez Y, Kolbe M, Erhardt M, Methylation of Salmonella Typhimurium flagella promotes bacterial adhesion and host cell invasion. Nat Commun, 11(1):2013(2020) [pubmed]

submitter keyword: Salmonella, host-pathogen interaction, post-translational modification, protein methylation

Marc Erhardt
contact affiliation	Humboldt-Universität zu Berlin, Institute for Biology – Bacterial Physiology, Berlin, Germany
contact email	marc.erhardt@hu-berlin.de
lab head
Christoph Krisp
contact affiliation	University Medical Center Hamburg-Eppendorf Institute of Clinical Chemistry and Laboratory Medicine Mass Spectrometric Proteomics
contact email	c.krisp@uke.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD017935

PRIDE project URI

• PRIDE
  1. PXD017935
     1. Label: PRIDE project
     2. Name: Methylation of Salmonella Typhimurium flagella promotes bacterial adhesion and host cell invasion