PXD022770

PXD022770 is an original dataset announced via ProteomeXchange.

Title	Spore formation in a parasitic cnidarian: proteomic analysis of Ceratonova shasta (Cnidaria: Myxozoa)
Description	Myxozoans are widely distributed aquatic obligate endoparasites that were recently recognized as belonging within the phylum Cnidaria. They have complex life cycles with waterborne transmission stages: resistant, infectious spores that are unique to myxozoans. However, little is known about the processes that give rise to these transmission stages. To understand the molecular underpinnings of spore formation, we conducted proteomics on Ceratonova shasta, a highly pathogenic myxozoan that causes severe mortalities in wild and hatchery-reared salmonid fishes. We compared proteomic profiles between developmental stages from inside the fish host, and the mature myxospore, which is released into the water where it drifts passively, ready to infect the next host. We found that C. shasta contains 2123 proteins; representing the first proteomic catalog of a myxozoan myxospore. Analysis of proteins differentially expressed between developing and mature spore stages uncovered processes that are active during spore formation. Our data highlight dynamic changes in the actin cytoskeleton, which provides myxozoan developmental stages with mobility through lamellipodia and filopodia, whereas in the mature myxospore the actin network supports F-actin stabilization that reinforces the transmission stage. These findings provide molecular insight into the myxozoan life cycle stages and, particularly, into the process of sporogenesis.
HostingRepository	PRIDE
AnnounceDate	2021-04-06
AnnouncementXML	Submission_2021-04-05_23:11:51.659.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD022770
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Tal BioInfo
SpeciesList	scientific name: Ceratonova shasta; NCBI TaxID: 60662;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-26 06:19:48	ID requested
⏵ 1	2021-04-05 23:11:56	announced

Dataset with its publication pending

submitter keyword: Cnidaria, Myxozoa, parasite, sporogenesis, cytoskeleton, actin

Tamar Lotan
contact affiliation	Marine Biology Department, The Leon H.Charney School of Marine Sciences, University of Haifa, Israel
contact email	lotant@univ.haifa.ac.il
lab head
Tal BioInfo
contact affiliation	University of Haifa
contact email	kaktust.uni@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD022770

PRIDE project URI

• PRIDE
  1. PXD022770
     1. Label: PRIDE project
     2. Name: Spore formation in a parasitic cnidarian: proteomic analysis of Ceratonova shasta (Cnidaria: Myxozoa)