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DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2014-07-25
  • AnnouncementXML: Submission_2014-07-25_00:41:34.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Hesse Anne-Marie
  • Title: SLP76 interactome in resting and activated primary mast cells
  • Description: We used a knock-in (KI) mouse expressing SLP-76 with a C-terminal One-Strep-Tag (OST). Such a system allowed the replacement in the genome of SLP-76 by its tagged version and its expression in physiological amounts. Then, by combining high affinity purification and label-free quantitative proteomics, we report here the first description of the SLP76 interactome in resting and activated primary cultured mast cells derived from mice expressing an OST at the C-terminus of SLP-76 (Slp76OST/OST mice).
  • SpeciesList: scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
  • ModificationList: deamidated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
  • Instrument: LTQ Orbitrap Velos

Dataset History

VersionDatetimeStatusChangeLog Entry
02012-10-16 05:23:40ID requested
12013-08-28 06:24:10announced
22013-08-28 06:27:18announcedAdd reference
12014-07-25 00:41:34announced

Publication List

  1. Bounab Y, Hesse AM, Iannascoli B, Grieco L, Couté Y, Niarakis A, Roncagalli R, Lie E, Lam KP, Demangel C, Thieffry D, Garin J, Malissen B, Daëron M, Proteomic analysis of the SH2 domain-containing leukocyte protein of 76 kDa (SLP76) interactome in resting and activated primary mast cells [corrected]. Mol Cell Proteomics, 12(10):2874-89(2013) [pubmed]

Keyword List

  1. submitter keyword: interactomics, SLP76, quantitative mass spectrometry

Contact List

    Hesse Anne-Marie
    • contact affiliation: Laboratoire d'Etude de la Dynamique des Proteomes
    • contact email: anne-marie.hesse@cea.fr
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List
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