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PXD000231

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2014-07-25
  • AnnouncementXML: Submission_2014-07-25_01:06:26.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Lancia Darville
  • Title: Mouse Cochlea Sensory Epithelium Proteome
  • Description: The mouse cochlear sensory epithelium proteome has been characterized using FASP combined with GELFrEE and off-line SCX- and WAX-based methods follwed by nano LC-MS/MS. In addition, we utilzed single digestion and double digestion approaches using LysC and trypsin endoproteases. MS data files were processed with MaxQuant (Version 1.2.2.5, Max Planck Institute) and peak list files were searched by MASCOT search engine against the UniProt mouse database containing both forward and reversed protein sequences and common contaminants such as keratin. The initial parent and fragment ion maximum precursors were set to 6 ppm and 0.5 Da, respectively. The search included a fixed modification of carbamidomethyl of cysteine and variable modifications of oxidation of methionine and protein N-terminal acetylation. The minimum peptide length to be considered for identification was 6 amino acids.
  • SpeciesList: scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
  • ModificationList: monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
  • Instrument: LTQ Orbitrap

Dataset History

VersionDatetimeStatusChangeLog Entry
02013-04-26 07:55:15ID requested
12013-06-28 03:55:10announced
22013-06-28 04:09:08announcedAdd reference
12014-07-25 01:06:26announced

Publication List

  1. Darville LN, Sokolowski BH, In-depth proteomic analysis of mouse cochlear sensory epithelium by mass spectrometry. J Proteome Res, 12(8):3620-30(2013) [pubmed]

Keyword List

  1. submitter keyword: Cochlea, FASP, nano LC-MS/MS, Sensory epithelium

Contact List

    Lancia Darville
    • contact affiliation: USF Dept. of Otolaryngology
    • contact email: ldarvill@health.usf.edu
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List
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