PXD000231
PXD000231 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Mouse Cochlea Sensory Epithelium Proteome |
| Description | The mouse cochlear sensory epithelium proteome has been characterized using FASP combined with GELFrEE and off-line SCX- and WAX-based methods follwed by nano LC-MS/MS. In addition, we utilzed single digestion and double digestion approaches using LysC and trypsin endoproteases. MS data files were processed with MaxQuant (Version 1.2.2.5, Max Planck Institute) and peak list files were searched by MASCOT search engine against the UniProt mouse database containing both forward and reversed protein sequences and common contaminants such as keratin. The initial parent and fragment ion maximum precursors were set to 6 ppm and 0.5 Da, respectively. The search included a fixed modification of carbamidomethyl of cysteine and variable modifications of oxidation of methionine and protein N-terminal acetylation. The minimum peptide length to be considered for identification was 6 amino acids. |
| HostingRepository | PRIDE |
| AnnounceDate | 2013-06-28 |
| AnnouncementXML | Submission_2013-06-28_04:09:08.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Lancia Darville |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | LTQ Orbitrap |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2013-04-26 07:55:15 | ID requested | |
| 1 | 2013-06-28 03:55:10 | announced | |
| 1 | 2014-07-25 01:06:26 | announced | |
| ⏵ 2 | 2013-06-28 04:09:08 | announced | Add reference |
Publication List
| Darville LN, Sokolowski BH, In-depth proteomic analysis of mouse cochlear sensory epithelium by mass spectrometry. J Proteome Res, 12(8):3620-30(2013) [pubmed] |
Keyword List
| submitter keyword: Cochlea, FASP, nano LC-MS/MS, Sensory epithelium |
Contact List
| Lancia Darville | |
|---|---|
| contact affiliation | USF Dept. of Otolaryngology |
| contact email | ldarvill@health.usf.edu |
Full Dataset Link List
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/2013/06/PXD000231 |
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