<< Full experiment listing

PXD001203

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2014-11-10
  • AnnouncementXML: Submission_2014-11-10_03:46:27.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Richard Scheltema
  • Title: The Q Exactive HF, a Benchtop Mass Spectrometer with a Pre-filter, High Performance Quadrupole and an Ultra-High Field Orbitrap Analyzer
  • Description: The quadrupole Orbitrap mass spectrometer (Q Exactive) has made a powerful proteomics instrument available in a benchtop format. It significantly boosted analyzable proteins per hour and has now evolved into a proteomics analysis workhorse for many laboratories. Here we describe the Q Exactive Plus and Q Exactive HF mass spectrometers, which feature several innovations in comparison to the original Q Exactive instrument. A low resolution pre-filter has been implemented within the injection flatapole, preventing unwanted ions from entering deep into the system, and thereby increasing its robustness. A new segmented quadrupole, with higher fidelity of isolation efficiency over a wide range of isolation windows, provides an almost two-fold improvement of transmission at narrow isolation widths. Additionally, the Q Exactive HF has a compact Orbitrap analyzer, leading to higher field strength and almost doubling resolution at the same transient times. With its very fast isolation and fragmentation capabilities, the instrument achieves overall cycle times of 1 s for a top 15 to 20 HCD (Higher energy Collisional Dissociation) method. We demonstrate identification of 5,000 proteins in standard 90-min gradients of tryptic digests of mammalian cell lysate, an increase of over 40% for detected peptides and over 20% for detected proteins. Additionally, we tested the instrument on peptide phosphorylation enriched samples, for which an improvement of up to 60% class I sites was observed.
  • SpeciesList: scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
  • ModificationList: No PTMs are included in the dataset
  • Instrument: Q Exactive

Dataset History

VersionDatetimeStatusChangeLog Entry
02014-08-06 01:33:43ID requested
12014-11-10 03:46:29announced

Publication List

  1. Scheltema RA, Hauschild JP, Lange O, Hornburg D, Denisov E, Damoc E, Kuehn A, Makarov A, Mann M, The Q Exactive HF, a Benchtop mass spectrometer with a pre-filter, high-performance quadrupole and an ultra-high-field Orbitrap analyzer. Mol Cell Proteomics, 13(12):3698-708(2014) [pubmed]

Keyword List

  1. curator keyword: Technical
  2. submitter keyword: Q Exactive Plus, Q Exactive HF, Orbitrap ultra-high field, segmented quadrupole, quadrupole pre-filter, Shotgun proteomics

Contact List

    Matthias Mann
    • contact affiliation: Max Planck Institute of Biochemistry Department for Proteomics and Signal Transduction Am Klopferspitz 18 D-82152 Martinsried
    • contact email: mann@biochem.mpg.de
    • lab head:
    Richard Scheltema
    • contact affiliation: MPI for Biochemistry
    • contact email: scheltem@biochem.mpg.de
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List
[+]
Subscribe to receive all new ProteomeXchange announcements!
If you have a question or comment about ProteomeXchange, please contact us!