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DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2015-07-20
  • AnnouncementXML: Submission_2015-07-20_05:31:01.xml
  • DigitalObjectIdentifier: http://dx.doi.org/10.6019/PXD001809
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Supported dataset by repository
  • PrimarySubmitter: Jon Humphries
  • Title: Mesenchymal stem cell integrin-associated complexes
  • Description: The composition and stiffness of the extracellular matrix (ECM) environment that surrounds Multipotent mesenchymal stem cells (MSCs) stem cells dictates transcriptional programming, thereby affecting stem cell lineage decision-making. Cells sense force between themselves and their microenvironment controlling the capability of MSCs to differentiate into adipocytes, osteocytes and chondrocytes. Force sensing is transmitted by integrin receptors and their associated adhesion signalling complexes. To identify regulators of MSC force sensing we sought to catalogue MSC adhesion complex composition. Therefore we isolated integrin-associated adhesion complexes formed in MSCs plated on the ECM ligand fibronectin. We identifed proteins using mass spectrometry that define a MSC specific subset of adhesion complex proteins consisting of key linkages to the actin cytoskeleton together with integrin signalling and force sensing components.
  • SpeciesList: scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
  • ModificationList: Oxidation; Carbamidomethyl
  • Instrument: LTQ Velos; LTQ Orbitrap Elite

Dataset History

VersionDatetimeStatusChangeLog Entry
02015-02-12 08:19:42ID requested
12015-07-20 05:31:02announced

Publication List

  1. Ajeian JN, Horton ER, Astudillo P, Byron A, Askari JA, Millon-Frémillon A, Knight D, Kimber SJ, Humphries MJ, Humphries JD, Proteomic analysis of integrin-associated complexes from mesenchymal stem cells. Proteomics Clin Appl, 10(1):51-7(2016) [pubmed]

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: MSC, Integrin, focal adhesions, Extracellular matrix, cell adhesion

Contact List

    Martin J Humphries
    • contact affiliation: Faculty of Life Sciences, University of Manchester, UK
    • contact email: martin.humphries@manchester.ac.uk
    • lab head:
    Jon Humphries
    • contact affiliation: The University of Manchester
    • contact email: j.d.humphries@manchester.ac.uk
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List
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