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PXD001809 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMesenchymal stem cell integrin-associated complexes
DescriptionThe composition and stiffness of the extracellular matrix (ECM) environment that surrounds Multipotent mesenchymal stem cells (MSCs) stem cells dictates transcriptional programming, thereby affecting stem cell lineage decision-making. Cells sense force between themselves and their microenvironment controlling the capability of MSCs to differentiate into adipocytes, osteocytes and chondrocytes. Force sensing is transmitted by integrin receptors and their associated adhesion signalling complexes. To identify regulators of MSC force sensing we sought to catalogue MSC adhesion complex composition. Therefore we isolated integrin-associated adhesion complexes formed in MSCs plated on the ECM ligand fibronectin. We identifed proteins using mass spectrometry that define a MSC specific subset of adhesion complex proteins consisting of key linkages to the actin cytoskeleton together with integrin signalling and force sensing components.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterJon Humphries
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListOxidation; Carbamidomethyl
InstrumentLTQ Velos; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-02-12 08:19:42ID requested
12015-07-20 05:31:02announced
Publication List
Ajeian JN, Horton ER, Astudillo P, Byron A, Askari JA, Millon-Frémillon A, Knight D, Kimber SJ, Humphries MJ, Humphries JD, Proteomic analysis of integrin-associated complexes from mesenchymal stem cells. Proteomics Clin Appl, 10(1):51-7(2016) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: MSC, Integrin, focal adhesions, Extracellular matrix, cell adhesion
Contact List
Martin J Humphries
contact affiliationFaculty of Life Sciences, University of Manchester, UK
contact emailmartin.humphries@manchester.ac.uk
lab head
Jon Humphries
contact affiliationThe University of Manchester
contact emailj.d.humphries@manchester.ac.uk
dataset submitter
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