PXD002069

PXD002069 is an original dataset announced via ProteomeXchange.

Title	Proteome imprinting by N-terminal acetylation is a vital hormone-regulated master switch during stress
Description	N-terminal acetylation (NTA) catalyzed by N-terminal acetyltransferases (Nats) is among the most common protein modifications in eukaryotes, but its significance is still enigmatic. Characterization of the plant NatA complex reveals evolutionary conservation of NatA biochemical properties within higher eukaryotes and uncovers specific and essential functions of NatA for regulation of development, numerous biosynthetic pathways and stress responses in plants. We show that NTA decreases significantly in case of drought stress, since NatA abundance is rapidly down-regulated by the phytohormone abscisic acid. Accordingly, down-regulation of NatA by genetic engineering is sufficient for induction of the drought stress response and results in strikingly drought resistant plants. Thus, NTA by the NatA complex is a vital cellular surveillance mechanism during stress. We conclude that imprinting of the proteome by NatA is a master switch for control of metabolism, development and cellular stress responses and integrates stress signals by perceiving the hormone abscisic acid.
HostingRepository	PRIDE
AnnounceDate	2016-06-14
AnnouncementXML	Submission_2016-06-14_07:32:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Willy Bienvenut
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue; acetate labeling reagent (N-term) (heavy form: +3amu)
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-04-15 01:11:51	ID requested
⏵ 1	2016-06-14 07:32:12	announced

Linster E, Stephan I, Bienvenut WV, Maple-Gr, ø, dem J, Myklebust LM, Huber M, Reichelt M, Sticht C, M, ø, ller SG, Meinnel T, Arnesen T, Giglione C, Hell R, Wirtz M, Downregulation of N-terminal acetylation triggers ABA-mediated drought responses in Arabidopsis. Nat Commun, 6():7640(2015) [pubmed]

curator keyword: Biological

submitter keyword: N-terminal protein acetylation, embryogenesis, drought resistance, abscisic acid

Willy Bienvenut
contact affiliation	I2BC, CNRS, Gif/Yvette, France
contact email	willy.bienvenut@isv.cnrs-gif.fr
lab head
Willy Bienvenut
contact affiliation	CNRS
contact email	willy.bienvenut@isv.cnrs-gif.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/06/PXD002069

PRIDE project URI

• PRIDE
  1. PXD002069
     1. Label: PRIDE project
     2. Name: Proteome imprinting by N-terminal acetylation is a vital hormone-regulated master switch during stress