PXD003634

PXD003634 is an original dataset announced via ProteomeXchange.

Title	An up to date workflow for plant (phospho)proteomics
Description	Protein phosphorylation is one of the most common post-translational modifications (PTMs), which can regulate protein activity and localization, as well as protein–protein interactions in numerous cellular processes. Phosphopeptide enrichment techniques enabled plant researchers to acquire insight in phosphorylation-controlled signaling networks in various plant species. However, most phosphoproteome analyses of plant samples still involve stable isotope labeling, peptide fractionation, and demand lots of mass spectrometry (MS) time. Here, we present a simple workflow to probe, map and catalogue plant phosphoproteomes, requiring low starting materials, no labeling, no fractionation, and limited analysis time. Following optimization of the different experimental steps on Arabidopsis material, we transferred our workflow to maize, a major monocot crop, to study stress signaling upon drought.
HostingRepository	PRIDE
AnnounceDate	2016-09-26
AnnouncementXML	Submission_2016-09-26_08:31:21.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Elisabeth Stes
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; scientific name: Zea mays (Maize); NCBI TaxID: 4577;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-02-15 01:53:45	ID requested
⏵ 1	2016-09-26 08:31:22	announced

Vu LD, Stes E, Van Bel M, Nelissen H, Maddelein D, Inz, é D, Coppens F, Martens L, Gevaert K, De Smet I, Up-to-Date Workflow for Plant (Phospho)proteomics Identifies Differential Drought-Responsive Phosphorylation Events in Maize Leaves. J Proteome Res, 15(12):4304-4317(2016) [pubmed]

curator keyword: Biological

submitter keyword: Maize, Arabidopsis, phosphoproteomics

Ive De Smet
contact affiliation	Ghent University VIB
contact email	ive.desmet@psb.vib-ugent.be
lab head
Elisabeth Stes
contact affiliation	VIB/UGent
contact email	elisabeth.stes@vib-ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/09/PXD003634

PRIDE project URI

• PRIDE
  1. PXD003634
     1. Label: PRIDE project
     2. Name: An up to date workflow for plant (phospho)proteomics