PXD004657

PXD004657 is an original dataset announced via ProteomeXchange.

Title	External and intrinsic mechanical stress restricts pheromone signaling by direct phosphorylation of the Ste5 scaffold by Pkc1
Description	Ste5 and Far1 function as prototypical scaffold proteins activating a MAP-kinase cascade and polarizing yeast cells in response to pheromones. Here we show that Pkc1-dependent phosphorylation of conserved serine residues in their RING-H2 domains down-regulates pheromone induced signaling upon mechanical stress. Phosphorylation of Ste5 on serine 185 by Pkc1 interferes with its membrane-association in vivo by preventing binding to the receptor linked Gbg protein as determined by in vitro assays. Quantitative readouts of pheromone signaling show that Pkc1 induces rapid dispersal of Ste5 from mating projections upon mechanically-induced cell wall stress as well as during cell-cell fusion, leading to inhibition of MAPK activity and polarized growth. Cells expressing non-phosphorylatable Ste5 (Ste5S185A) undergo increased lysis during mechanical stress and cell-cell fusion compared to wild-type controls, and this effect is exacerbated by simultaneous expression of non-phosphorylatable Far1 (Far13A). Taken together, these results uncover a cross-talk mechanism explaining how mating cells inhibit pheromone signaling upon extrinsic or intrinsic mechanical stress to orchestrate polarized growth and cell-cell fusion.
HostingRepository	PRIDE
AnnounceDate	2019-06-17
AnnouncementXML	Submission_2019-06-17_05:46:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wolfgang Reiter
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	LTQ; LTQ FT; LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-07-26 06:54:46	ID requested
⏵ 1	2019-06-17 05:46:09	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Yeast, Saccharomyces cerevisiae, MAPK, scaffold, Ste5, Far1

Wolfgang Reiter
contact affiliation	Department of Biochemistry, Max F. Perutz Laboratories, University of Vienna, Dr. Bohrgasse 9, 1030 Vienna, Austria
contact email	wolfgang.l.reiter@univie.ac.at
lab head
Wolfgang Reiter
contact affiliation	MAX F. PERUTZ LABORATORIES - University of Vienna
contact email	wolfgang.l.reiter@univie.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD004657
     1. Label: PRIDE project
     2. Name: External and intrinsic mechanical stress restricts pheromone signaling by direct phosphorylation of the Ste5 scaffold by Pkc1