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PXD008156

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2019-02-18
  • AnnouncementXML: Submission_2019-02-18_07:23:56.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Maria Sebastião
  • Title: Human myocardial ischemia/reperfusion injury model to study cardiac stem cells activation and regeneration mechanisms
  • Description: The importance of regenerative potential of cardiac stem/progenitor cells (CSCs) after acute myocardial infarction (AMI) has been emphasized in the last decade. Several studies have demonstrated that this cell population has a relevant role in myocardial repair, supported by the establishment of a paracrine cross-talk between CSCs and the injured tissue. However, the majority of current in vitro strategies to study these cells fails to include important modulators of CSC activity, such as the paracrine effect of cardiomyocytes (CMs) and the physicochemical changes occurring in tissue during Ischemia/Reperfusion (I/R) injury. In this work, we established the first human in vitro heterotypic model of myocardial I/R injury with human CSCs (hCSCs) and human induced pluripotent cell-derived cardiomyocytes (hiPSC-CMs) using transwell inserts. hCSCs response to I/R was characterized using advanced whole proteomic mass spectrometry-based tools that allowed us to propose new pathways in hCSCs regenerative process, including: cell cycle regulation inhibition, proliferation through EGF signaling, gluthathione-mediated reactive oxygen species detoxification, and signaling through several paracrine growth factors. The model established in this work contributes with new insights regarding hCSC biology in response to AMI and provides an important tool to study and modulate molecular mechanisms involved in the regenerative potential of CSCs.
  • SpeciesList: scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
  • ModificationList: monohydroxylated residue; iodoacetamide derivatized residue
  • Instrument: TripleTOF 6600

Dataset History

VersionDatetimeStatusChangeLog Entry
02017-11-07 07:44:39ID requested
12019-02-18 07:23:57announced

Publication List

  1. Dataset with its publication pending

Keyword List

  1. curator keyword: Biological, Biomedical
  2. submitter keyword: cardiac stem/progenitor cells
  3. acute myocardial infarction
  4. ischemia/reperfusion injury

Contact List

    Paula Marques Alves
    • contact affiliation: ITQB-NOVA, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. Da República, 2780-157 Oeiras, Portugal;iBET, Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2780-901, Oeiras , Portugal
    • contact email: marques@ibet.pt
    • lab head:
    Maria Sebastião
    • contact affiliation: iBET/ ITQB-NOVA
    • contact email: msebastiao@itqb.unl.pt
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List
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