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DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2019-02-18
  • AnnouncementXML: Submission_2019-02-18_06:00:39.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Katarzyna Dolata
  • Title: Dynamics of the Escherichia coli proteome in response to the deletion of the Tat pathway
  • Description: In Escherichia coli, the Twin-arginine (Tat) secretion system is one of the main routes of the protein export to the periplasm. The Tat pathway secretes a set of proteins with important physiological functions. In our study, we investigated the influence of the deactivation of the Tat pathway on the E. coli cells. We applied a comprehensive and comparative proteomic analysis of the E. coli wild type and tat mutant. This dataset provides mass spectrometry based details on the abundances of proteins in cytoplasmic, periplasmic and membrane fractions. We observed that a tat deletion increases abundances of proteins involved in protein folding, degradation, responses to heat, oxidation, osmolarity, and cold. Moreover, the impairment of E. coli outer membrane resulted in the activation of proteins responsible for cell wall biogenesis. The tat deletion negatively affects the synthesis of iron transporters and imbalances its homeostasis in the cell.
  • SpeciesList: scientific name: Escherichia coli; NCBI TaxID: 562;
  • ModificationList: No PTMs are included in the dataset
  • Instrument: Synapt MS; LTQ Orbitrap Elite

Dataset History

VersionDatetimeStatusChangeLog Entry
02018-01-30 08:43:31ID requested
12019-02-18 06:00:40announced

Publication List

  1. Dolata KM, Montero IG, Miller W, Sievers S, Sura T, Wolff C, Schlüter R, Riedel K, Robinson C, Far-reaching cellular consequences of tat deletion in Escherichia coli revealed by comprehensive proteome analyses. Microbiol Res, 218():97-107(2019) [pubmed]

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: Escherichia coli, Stress responses, Tat pathway

Contact List

    Katarzyna Dolata
    • contact affiliation: Institute of Microbiology, Department of Microbial Physiology and Molecular Biology Ernst-Moritz-Arndt-University Greifswald Felix-Hausdorff-Straße 8 17489 Greifswald
    • contact email: dolatak@uni-greifswald.de
    • lab head:
    Katarzyna Dolata
    • contact affiliation: University of Greifswald
    • contact email: dolatak@uni-greifswald.de
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List

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