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PXD008803

PXD008803 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDynamics of the Escherichia coli proteome in response to the deletion of the Tat pathway
DescriptionIn Escherichia coli, the Twin-arginine (Tat) secretion system is one of the main routes of the protein export to the periplasm. The Tat pathway secretes a set of proteins with important physiological functions. In our study, we investigated the influence of the deactivation of the Tat pathway on the E. coli cells. We applied a comprehensive and comparative proteomic analysis of the E. coli wild type and tat mutant. This dataset provides mass spectrometry based details on the abundances of proteins in cytoplasmic, periplasmic and membrane fractions. We observed that a tat deletion increases abundances of proteins involved in protein folding, degradation, responses to heat, oxidation, osmolarity, and cold. Moreover, the impairment of E. coli outer membrane resulted in the activation of proteins responsible for cell wall biogenesis. The tat deletion negatively affects the synthesis of iron transporters and imbalances its homeostasis in the cell.
HostingRepositoryPRIDE
AnnounceDate2019-02-18
AnnouncementXMLSubmission_2019-02-18_06:00:39.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKatarzyna Dolata
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListNo PTMs are included in the dataset
InstrumentSynapt MS; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-01-30 08:43:31ID requested
12019-02-18 06:00:40announced
Publication List
Dolata KM, Montero IG, Miller W, Sievers S, Sura T, Wolff C, Schl, ü, ter R, Riedel K, Robinson C, Far-reaching cellular consequences of tat deletion in Escherichia coli revealed by comprehensive proteome analyses. Microbiol Res, 218():97-107(2019) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Escherichia coli, Stress responses, Tat pathway
Contact List
Katarzyna Dolata
contact affiliationInstitute of Microbiology, Department of Microbial Physiology and Molecular Biology Ernst-Moritz-Arndt-University Greifswald Felix-Hausdorff-Straße 8 17489 Greifswald
contact emaildolatak@uni-greifswald.de
lab head
Katarzyna Dolata
contact affiliationUniversity of Greifswald
contact emaildolatak@uni-greifswald.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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