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PXD009294

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2019-01-09
  • AnnouncementXML: Submission_2019-01-14_04:40:47.xml
  • DigitalObjectIdentifier: http://dx.doi.org/10.6019/PXD009294
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Supported dataset by repository
  • PrimarySubmitter: Virginie Redeker
  • Title: Identification of proteins that interact with extracellularly applied fibrillar Tau assemblies
  • Description: The microtubule associated protein Tau (MAPT) expressed in neurons is involved in microtubules stabilization, cell morphogenesis and axonal transport. In pathological conditions, Tau assembles into high molecular weight assemblies leading to neuropathological Tau deposits, the hallmark of several neurodegenerative diseases collectively named “tauopathies”, including Alzheimer’s disease. These pathologic Tau assemblies are released by affected neuronal cells and taken up by naïve neighbor cells, propagate from one cell to another and amplify by seeding the aggregation of endogenous Tau. In order to identify plasma membrane proteins exposed extracellularly that interact with extracellularly applied fibrillar Tau assemblies, we exposed pure-neuronal cultures to fibrillar Tau for 10 min, pulled down the associated proteins, and identified them using a proteomic-based approach. Of the six Tau isoforms produced by alternative splicing of the MAPT gene and differing from each other by the presence or absence of one or two inserts in the N-terminal part (0N, 1N or 2N) of the protein and by the presence of either three or four repeated microtubule binding motifs in the protein C-terminal part (3R or 4R), we have expressed and purified 1N3R and 1N4R Tau isoforms and further assembled them into 1N3R and 1N4R Tau fibrils. Using pull-down of whole cell lysates and mass spectrometry, we have identified proteins interacting with extracellularly applied Tau fibrils. We have performed two different experiments with either 1N3R Tau fibrils or 1N4R Tau fibrils (condition 1 and condition 2 respectively). Each condition consists in six experimental replicates of cells exposed 10 min to Tau fibrils and of the non-treated cells used as controls.
  • SpeciesList: scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
  • ModificationList: Glu->pyro-Glu; Oxidation; Acetyl; Carbamidomethyl; Gln->pyro-Glu
  • Instrument: TripleTOF 4600

Dataset History

VersionDatetimeStatusChangeLog Entry
02018-03-22 02:36:35ID requested
12019-01-09 08:39:31announced
22019-01-14 04:40:49announcedUpdated publication reference for PubMed record(s): 30630857.

Publication List

  1. Shrivastava AN, Redeker V, Pieri L, Bousset L, Renner M, Madiona K, Mailhes-Hamon C, Coens A, Buée L, Hantraye P, Triller A, Melki R, -ATPase and AMPA receptors. EMBO J, 38(3):(2019) [pubmed]

Keyword List

  1. curator keyword: Biological, Biomedical
  2. submitter keyword: Tau fibrils, 1N3R isoform, 1N4R isoform, Interacting protein partners, Pull-down, LC-MSMS, Proteomics

Contact List

    Ronald Melki, Virginie Redeker
    • contact affiliation: Paris-Saclay Institute of Neuroscience, and CEA, Institut François Jacob, Université Paris-Saclay, Team Melki, CNRS, Gif-sur-Yvette, France
    • contact email: virginie.redeker@cnrs.fr
    • lab head:
    Virginie Redeker
    • contact affiliation: CNRS
    • contact email: virginie.redeker@cnrs.fr
    • dataset submitter:

Full Dataset Link List

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