PXD010078

PXD010078 is an original dataset announced via ProteomeXchange.

Title	Analysis of Escherichia coli CyDisCo proteome changes in response to the expression of scFv and the misfolded scFv
Description	E. coli CyDisCo strain enables a high yield secretion of disulfide bond-containing proteins to the periplasm via Twin-arginine (Tat) pathway. Introducing two exogenous oxidases: the yeast sulfydryl oxidase (Erv1p) and human protein disulfide isomerase (PDI), the CyDisCo strain changes the cytoplasm into an oxidized environment, where the disulfide bonds can efficiently be formed. In this study, we analyzed the proteome changes upon the expression of disulfide bond-containing scFv and the misfolded scFv in the CyDisCo strain. The correctly folded protein is secreted to the periplasm, while the misfolded protein accumulates exclusively in the inclusion body fraction. We observed a high number of significant changes mostly in proteins associated with protein folding and degradation, oxidative stress, membrane transport and integrity.
HostingRepository	PRIDE
AnnounceDate	2019-02-18
AnnouncementXML	Submission_2019-02-18_06:43:57.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Katarzyna Dolata
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-11 00:13:19	ID requested
⏵ 1	2019-02-18 06:43:58	announced

Guerrero Montero I, Dolata KM, Schl, ü, ter R, Malherbe G, Sievers S, Z, ü, hlke D, Sura T, Dave E, Riedel K, Robinson C, Comparative proteome analysis in an Escherichia coli CyDisCo strain identifies stress responses related to protein production, oxidative stress and accumulation of misfolded protein. Microb Cell Fact, 18(1):19(2019) [pubmed]

curator keyword: Biological

submitter keyword: Escherichia coli, Tat system, Proteome, Stress response

Katarzyna Dolata
contact affiliation	Institute of Microbiology, Department of Microbial Physiology and Molecular Biology Ernst-Moritz-Arndt-University Greifswald Felix-Hausdorff-Straße 8 17489 Greifswald
contact email	dolatak@uni-greifswald.de
lab head
Katarzyna Dolata
contact affiliation	University of Greifswald
contact email	dolatak@uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010078
     1. Label: PRIDE project
     2. Name: Analysis of Escherichia coli CyDisCo proteome changes in response to the expression of scFv and the misfolded scFv