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PXD010078

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2019-02-18
  • AnnouncementXML: Submission_2019-02-18_06:43:57.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Katarzyna Dolata
  • Title: Analysis of Escherichia coli CyDisCo proteome changes in response to the expression of scFv and the misfolded scFv
  • Description: E. coli CyDisCo strain enables a high yield secretion of disulfide bond-containing proteins to the periplasm via Twin-arginine (Tat) pathway. Introducing two exogenous oxidases: the yeast sulfydryl oxidase (Erv1p) and human protein disulfide isomerase (PDI), the CyDisCo strain changes the cytoplasm into an oxidized environment, where the disulfide bonds can efficiently be formed. In this study, we analyzed the proteome changes upon the expression of disulfide bond-containing scFv and the misfolded scFv in the CyDisCo strain. The correctly folded protein is secreted to the periplasm, while the misfolded protein accumulates exclusively in the inclusion body fraction. We observed a high number of significant changes mostly in proteins associated with protein folding and degradation, oxidative stress, membrane transport and integrity.
  • SpeciesList: scientific name: Escherichia coli; NCBI TaxID: 562;
  • ModificationList: acetylated residue; iodoacetamide derivatized residue
  • Instrument: Synapt MS; LTQ Orbitrap Elite

Dataset History

VersionDatetimeStatusChangeLog Entry
02018-06-11 00:13:19ID requested
12019-02-18 06:43:58announced

Publication List

  1. Guerrero Montero I, Dolata KM, Schlüter R, Malherbe G, Sievers S, Zühlke D, Sura T, Dave E, Riedel K, Robinson C, Comparative proteome analysis in an Escherichia coli CyDisCo strain identifies stress responses related to protein production, oxidative stress and accumulation of misfolded protein. Microb Cell Fact, 18(1):19(2019) [pubmed]

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: Escherichia coli, Tat system, Proteome, Stress response

Contact List

    Katarzyna Dolata
    • contact affiliation: Institute of Microbiology, Department of Microbial Physiology and Molecular Biology Ernst-Moritz-Arndt-University Greifswald Felix-Hausdorff-Straße 8 17489 Greifswald
    • contact email: dolatak@uni-greifswald.de
    • lab head:
    Katarzyna Dolata
    • contact affiliation: University of Greifswald
    • contact email: dolatak@uni-greifswald.de
    • dataset submitter:

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