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DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2019-02-18
  • AnnouncementXML: Submission_2019-02-18_05:36:54.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Tuula Nyman
  • Title: Estrogen Receptor alpha and Histone Lysine Methyltransferase DOT1L chromatin bound interactome
  • Description: Estrogen Receptor alpha (ERα) is a ligand-inducible transcription factor that mediates estrogen signaling in hormone-responsive breast cancer (BC) and is the primary target of specific anticancer therapies. Although ERα blockade with these drugs is effective, the development of a resistance to treatment represents the key problem in clinical management of patients affected by this disease. Understanding the molecular mechanisms underlying ERα action in BC cells may help the identification of new therapeutic targets for more effective pharmacological treatment of endocrine therapy-resistant tumors. We recently discovered the epigenetic enzyme DOT1L (DOT1 Like Histone Lysine Methyltransferase) as a novel nuclear partner of ERα in BC cells. To investigate the involvement of DOT1L in mediating ERα actions in hormone-responsive and endocrine-resistant BC, physical and functional interaction between these two molecules on chromatin was mapped by Chromatin Immunoprecipitation coupled to Mass Spectrometry (ChIP-MS).
  • SpeciesList: scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
  • ModificationList: iodoacetamide derivatized residue
  • Instrument: Q Exactive

Dataset History

VersionDatetimeStatusChangeLog Entry
02018-07-05 01:54:21ID requested
12019-02-18 05:36:55announced

Publication List

  1. Dataset with its publication pending

Keyword List

  1. curator keyword: Biological, Biomedical
  2. submitter keyword: estrogen signaling, estrogen receptor alpha, DOT1L, breast cancer chromatin interactome, LC-MS/MS

Contact List

    Tuula Nyman
    • contact affiliation: Head of Proteomics
    • contact email: tuula.nyman@medisin.uio.no
    • lab head:
    Tuula Nyman
    • contact affiliation: University of Oslo
    • contact email: tuula.nyman@medisin.uio.no
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List

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