PXD010314

PXD010314 is an original dataset announced via ProteomeXchange.

Title	Estrogen Receptor alpha and Histone Lysine Methyltransferase DOT1L chromatin bound interactome
Description	Estrogen Receptor alpha (ERα) is a ligand-inducible transcription factor that mediates estrogen signaling in hormone-responsive breast cancer (BC) and is the primary target of specific anticancer therapies. Although ERα blockade with these drugs is effective, the development of a resistance to treatment represents the key problem in clinical management of patients affected by this disease. Understanding the molecular mechanisms underlying ERα action in BC cells may help the identification of new therapeutic targets for more effective pharmacological treatment of endocrine therapy-resistant tumors. We recently discovered the epigenetic enzyme DOT1L (DOT1 Like Histone Lysine Methyltransferase) as a novel nuclear partner of ERα in BC cells. To investigate the involvement of DOT1L in mediating ERα actions in hormone-responsive and endocrine-resistant BC, physical and functional interaction between these two molecules on chromatin was mapped by Chromatin Immunoprecipitation coupled to Mass Spectrometry (ChIP-MS).
HostingRepository	PRIDE
AnnounceDate	2019-02-18
AnnouncementXML	Submission_2019-02-18_05:36:54.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tuula Nyman
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-05 01:54:21	ID requested
⏵ 1	2019-02-18 05:36:55	announced

Dataset with its publication pending

curator keyword: Biological, Biomedical

submitter keyword: estrogen signaling, estrogen receptor alpha, DOT1L, breast cancer chromatin interactome, LC-MS/MS

Tuula Nyman
contact affiliation	Head of Proteomics
contact email	tuula.nyman@medisin.uio.no
lab head
Tuula Nyman
contact affiliation	University of Oslo
contact email	tuula.nyman@medisin.uio.no
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010314
     1. Label: PRIDE project
     2. Name: Estrogen Receptor alpha and Histone Lysine Methyltransferase DOT1L chromatin bound interactome