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PXD010336

PXD010336 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIntegrated Dissection of Cysteine Oxidative Modification Proteome During Cardiac Hypertrophy
DescriptionCysteine oxidative modification of cellular proteins is crucial for many aspects of cardiac hypertrophy development. However, integrated dissection of multiple types of cysteine oxidation proteome in cardiac hypertrophy is currently missing. Here we developed a novel discovery platform encompassing a customized biotin switch-based quantitative proteomics pipeline and an advanced analytic workflow to comprehensively profile the landscape of cysteine oxidation in ISO-induced cardiac hypertrophy mouse model. Specifically, we identified a total of 3,717 proteins containing 6,837 oxidized cysteine sites by at least one of reversible cysteine oxidation, cysteine sulfinylation (CysSO2H), and cysteine sulfonylation (CysSO3H). Analyzing the hypertrophy signatures that are reproducibly discovered from computational workflow highlighted a group of fatty acid beta-oxidation enzymes with a continual decreased temporal pattern and a significant decreased abundance in reversible oxidation with no temporal or abundance change in total cysteine, revealing the oxidative regulatory map of fatty acid metabolism in cardiac hypertrophy, which is featured by an overall reduced oxidative metabolism. Our cysteine oxidation platform depicts a dynamic and integrated landscape of the cysteine oxidative proteome, extracted molecular signature, and provided mechanistic insights in cardiac hypertrophy.
HostingRepositoryPRIDE
AnnounceDate2018-08-29
AnnouncementXMLSubmission_2018-08-29_05:09:48.xml
DigitalObjectIdentifierhttp://dx.doi.org/10.6019/PXD010336
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterHoward Choi
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListbiotin-maleimide; Dimethyl; NEM; Carbamidomethyl; 47.9947; Dimethyl:2H(4)
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-07-06 09:08:44ID requested
12018-08-29 05:09:50announced
Publication List
Wang J, Choi H, Chung NC, Cao Q, Ng DCM, Mirza B, Scruggs SB, Wang D, Garlid AO, Ping P, Integrated Dissection of Cysteine Oxidative Post-translational Modification Proteome During Cardiac Hypertrophy. J Proteome Res, 17(12):4243-4257(2018) [pubmed]
Keyword List
submitter keyword: mouse, heart, cysteine oxidation, reversible cysteine oxidation, cysteine sulfinylation, cysteine sulfonylation, cardiac hypertrophy, biotin switch, dimethyl labeling, quantitative proteomics
Contact List
Peipei Ping
contact affiliationDavid Geffen School of Medicine, UCLA, USA BD2K Center of Excellence for Big Data Computing at UCLA, UCLA, USA
contact emailpping38@g.ucla.edu
lab head
Howard Choi
contact affiliationBD2K Center of Excellence for Big Data Computing at UCLA, UCLA, USA
contact emailcjh9595@g.ucla.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
PRIDE project URI
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