PXD010537

PXD010537 is an original dataset announced via ProteomeXchange.

Title	Phospho and expression proteomics in chinese hamster ovary cells
Description	We investigated CHO signaling events in a comparative expression and phospho proteomics approach of recombinant mAb producing XL99 cell line and according parental cells. First, on proteomic level differences between producer and parental cells were evaluated by label-free quantification of proteins at exponential growth. Next, a SILAC-based phosphoproteomic investigation was used to elucidate differential phosphorylation events following IGF treatment as well as differences between two producer cell lines and the parental cells.
HostingRepository	PRIDE
AnnounceDate	2020-02-26
AnnouncementXML	Submission_2020-02-26_07:30:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Louise Brachtvogel
SpeciesList	scientific name: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus); NCBI TaxID: 10029;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-24 00:00:03	ID requested
⏵ 1	2020-02-26 07:30:34	announced

Schelletter L, Albaum S, Walter S, Noll T, Hoffrogge R, Clonal variations in CHO IGF signaling investigated by SILAC-based phosphoproteomics and LFQ-MS. Appl Microbiol Biotechnol, 103(19):8127-8143(2019) [pubmed]

curator keyword: Biological

submitter keyword: LC-MSMS, SILAC, LFQ, CHO, Phosphoproteomics, Proteomics

Dr. Raimund Hoffrogge
contact affiliation	Cell culture technology, Technical faculty, Bielefeld University
contact email	raimund.hoffrogge@uni-bielefeld.de
lab head
Louise Brachtvogel
contact affiliation	Bielefeld University
contact email	l.brachtvogel@uni-bielefeld.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010537
     1. Label: PRIDE project
     2. Name: Phospho and expression proteomics in chinese hamster ovary cells