PXD010744

PXD010744 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic analysis of primary prostate cancer tissue specimens
Description	Currently, comprehensive and quantitative proteomic analysis of human prostate cancer tissue specimens remains scarce, hindering the identification of protein complexes and pathways deregulated in prostate cancer. In this study, we applied TMT-SPS-MS3-based quantitative proteomics to analzye 9 normal controls, 9 low-grade prostate cancer, and 9 high-grade prostate cancer. About 3,600 proteins were quantified across all the 27 prostate specimens. Statistical analysis identified 651 proteins that are differentially expressed in high-grade prostate cancer and normal prostate. Pathway enrichment analysis revealed that the LXR/RXR activation and integrin signaling pathways are substantially downregulated in high-grade prostate cancer, compared with normal prostate cancer. In addition, protein complex analysis suggested that mitochondrial ribosomes and ribosome-biogenesis complexes are significnatly overexpressed, whereas the cholesterol effluex and focal adhesion comlexes are significantly downregulated in high-grade prostate cancer, compared with normal controls. Furthermore, differential correlation analysis indicated that the spliceosome machinery might be more active in low-grade prostate cancer, compared with normal controls. The results are expected to shed light on the molecular mechnanisms underlying the development and progression of primary prostate cancer in human patients.
HostingRepository	PRIDE
AnnounceDate	2019-04-23
AnnouncementXML	Submission_2019-05-14_07:25:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei Yang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-10 02:15:23	ID requested
1	2019-04-23 04:09:25	announced
⏵ 2	2019-05-14 07:25:10	announced	Updated publication reference for PubMed record(s): 31011308.

Zhou B, Yan Y, Wang Y, You S, Freeman MR, Yang W, Quantitative proteomic analysis of prostate tissue specimens identifies deregulated protein complexes in primary prostate cancer. Clin Proteomics, 16():15(2019) [pubmed]

ProteomeXchange project tag: Cancer (B/D-HPP)

curator keyword: Biomedical

submitter keyword: prostate cancer, TMT, SPS-MS3, tissue, proteomics, differential expression analysis, differential correlation analysis, protein complex, pathway

Wei Yang
contact affiliation	Departments of Surgery and Biomedical Sciences Cedars-Sinai Medical Center
contact email	wei.yang@cshs.org
lab head
Wei Yang
contact affiliation	Cedars-Sinai Medical Center
contact email	omician@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010744
     1. Label: PRIDE project
     2. Name: Quantitative proteomic analysis of primary prostate cancer tissue specimens