PXD010819

PXD010819 is an original dataset announced via ProteomeXchange.

Title	Perception of the peptide hormone Systemin
Description	Cell suspension cultures of Solanum peruvianum were subjected to stimulation with 10nM systemin, the inactive systemin analog10nM a17, or injected with equal volume of water. For each treatment, a batch of cells was harvested after 2 minutes, 5 minutes, 15 minutes and 45 minutes. Untreated cells were harvested as controls. All treatments and time points were sampled as three independent replicates using independent sets of cells. Microsomal membranes were isolated from each sample, digested with trypsin, enriched for phosphopeptides and subjected to untargeted data-dependent acquisition by LC-MS/MS. Data analysis was performed jointly for all samples, and intensity values of triplicates were averaged.
HostingRepository	PRIDE
AnnounceDate	2019-06-17
AnnouncementXML	Submission_2019-06-17_06:43:57.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fatima Haj Ahmad
SpeciesList	scientific name: Solanum peruvianum; NCBI TaxID: 4082;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-20 02:16:31	ID requested
⏵ 1	2019-06-17 06:43:59	announced

Haj Ahmad F, Wu XN, Stintzi A, Schaller A, Schulze WX, The Systemin Signaling Cascade As Derived from Time Course Analyses of the Systemin-responsive Phosphoproteome. Mol Cell Proteomics, 18(8):1526-1542(2019) [pubmed]

curator keyword: Biological

submitter keyword: Systemin

Solanum peruvianum

membrane proteins

Phosphoproteomics

LC-MS/MS

Andreas Klaus Schaller
contact affiliation	Institute of Plant Physiology and Biotechnology University of Hohenheim
contact email	andreas.schaller@uni-hohenheim.de
lab head
Fatima Haj Ahmad
contact affiliation	Uninversity of Hohenheim. Institute of Plant Physiology and biotechnology
contact email	fatimahajahmad@yahoo.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD010819

PRIDE project URI

• PRIDE
  1. PXD010819
     1. Label: PRIDE project
     2. Name: Perception of the peptide hormone Systemin