PXD012226

PXD012226 is an original dataset announced via ProteomeXchange.

Title	Application of data-independent acquisition approach to study the proteome dynamics of plant pathogenesis responses
Description	Plants and pathogens are entangled in a continual arms race. The plants are evolved to have a dynamic defense and immune mechanisms to resist the infection and enhance the immunity for the second wave attacks from the same or different type of pathogenic species. Not only in the evolutionally or physiologically, the plant-pathogen interaction is also highly dynamic in the molecular level. Recently, the emerging quantitative mass spectrometry-based proteomics approach, data-independent acquisition (DIA), was developed for the analysis of proteome in a high throughput fashion. In this study, the DIA approach was applied to quantitatively trace the change of the plant proteome from the early to late stage of pathogenesis progression. This study revealed that the early stage of the pathogenesis response, the proteins directly related to the chaperon for the defense proteins. In the later stage, not only the defense proteins but also a set of the pathogen associate molecular pattern triggered immunity (PTI), effector triggered immunity (ETI) related proteins were highly induced. Our finding showed the dynamics of the regulation in protein level and demonstrated that the potential of using DIA approach for tracing the dynamics of the plant proteome during pathogenesis responses.
HostingRepository	PRIDE
AnnounceDate	2019-02-18
AnnouncementXML	Submission_2019-02-18_08:21:46.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei-Hung Chang
SpeciesList	scientific name: Solanum lycopersicum; NCBI TaxID: 4081;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2019-01-03 03:52:37	ID requested
⏵ 1	2019-02-18 08:21:47	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Plant pathogenesis responses, data-independent acquisition, quantitative proteomics, Pseudomonas syringae

Yet-Ran Chen
contact affiliation	Academia Sinica
contact email	yetran@gate.sinica.edu.tw
lab head
Wei-Hung Chang
contact affiliation	Academia Sinica
contact email	whchang@gate.sinica.edu.tw
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD012226

PRIDE project URI

• PRIDE
  1. PXD012226
     1. Label: PRIDE project
     2. Name: Application of data-independent acquisition approach to study the proteome dynamics of plant pathogenesis responses