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PXD012727

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2019-02-18
  • AnnouncementXML: Submission_2019-02-18_07:30:28.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Tamar Geiger
  • Title: Novel proteomic approach (PUNCH-P) reveals cell cycle-specific fluctuations in mRNA translation
  • Description: Monitoring protein synthesis is essential to our understanding of gene expression regulation, as protein abundance is thought to be predominantly controlled at the level of translation. Mass-spectrometric and RNA sequencing methods have been recently developed for investigating mRNA translation at a global level, but these still involve technical limitations and are not widely applicable. In this study, we describe a novel system-wide proteomic approach for direct monitoring of translation, termed puromycin-associated nascent chain proteomics (PUNCH-P), which is based on incorporation of biotinylated puromycin into newly synthesized proteins under cell-free conditions followed by streptavidin affinity purification and liquid chromatography-tandem mass spectrometry analysis. Using PUNCH-P, we measured cell cycle-specific fluctuations in synthesis for >5000 proteins in mammalian cells, identified proteins not previously implicated in cell cycle processes, and generated the first translational profile of a whole mouse brain. This simple and economical technique is broadly applicable to any cell type and tissue, enabling the identification and quantification of rapid proteome responses under various biological conditions.
  • SpeciesList: scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
  • ModificationList: No PTMs are included in the dataset
  • Instrument: Q Exactive

Dataset History

VersionDatetimeStatusChangeLog Entry
02019-02-15 03:23:47ID requested
12019-02-18 07:30:29announced

Publication List

  1. Aviner R, Geiger T, Elroy-Stein O, Novel proteomic approach (PUNCH-P) reveals cell cycle-specific fluctuations in mRNA translation. Genes Dev, 27(16):1834-44(2013) [pubmed]

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: Translatome, PUNCH-P, ribosome

Contact List

    Tamar Geiger
    • contact affiliation: Tel Aviv Universtiy
    • contact email: geiger@tauex.tau.ac.il
    • lab head:
    Tamar Geiger
    • contact affiliation: Tel Aviv University
    • contact email: geiger@tauex.tau.ac.il
    • dataset submitter:

Full Dataset Link List

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