PXD013039

PXD013039 is an original dataset announced via ProteomeXchange.

Title	Circadian analysis of the mouse cerebellum
Description	The cerebellum contains a circadian clock, generating internal temporal signals. The daily oscillations of cerebellar proteins were investigated here in mice using large-scale two-dimensional differences in gel electrophoresis (2D-DIGE). Analysis of 2D-DIGE gels highlighted the rhythmic variation in the intensity of 27/588 protein spots (5%) over 24-h based on cosinor regression. Notably, rhythmic expression of most abundant cerebellar proteins was clustered in two main phases (i.e., midday and midnight), leading to bimodal distribution. Only 6 proteins identified here to be rhythmic in the cerebellum are also known to oscillate in the suprachiasmatic nuclei, including 2 proteins involved in synapse activity (Synapsin 2 (Syn2) and vesicle-fusing ATPase (Nsf)), 2 others participating in carbohydrate metabolism (triosephosphate isomerase (Tpi1) and alpha-enolase (Eno1)), and Glutamine synthetase (Glul), as well as Tubulin alpha (Tuba4a). Most oscillating cerebellar proteins were not previously identified in circadian proteomic analyses of any tissue. Strikingly, daily accumulation of mitochondrial proteins was clustered to the mid-resting phase, as previously observed for distinct mitochondrial proteins in the liver. This study highlights extensive rhythmic aspects of the cerebellar proteome.
HostingRepository	PRIDE
AnnounceDate	2019-04-23
AnnouncementXML	Submission_2019-04-23_04:56:54.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fabrice BERTILE
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	maXis 4G; Synapt HDMS

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-11 04:24:56	ID requested
⏵ 1	2019-04-23 04:56:56	announced

Plumel M, Dumont S, Maes P, Sandu C, Felder-Schmittbuhl MP, Challet E, Bertile F, Circadian Analysis of the Mouse Cerebellum Proteome. Int J Mol Sci, 20(8):(2019) [pubmed]

curator keyword: Biological

submitter keyword: Mouse, cerebellum, circadian rhythm, LC-MSMS

Sarah Cianférani
contact affiliation	Laboratoire de Spectrométrie de Masse BioOrganique, Analytical Science department, IPHC, CNRS, Strasbourg University, UMR7178, Strasbourg, france
contact email	sarah.cianferani@unistra.fr
lab head
Fabrice BERTILE
contact affiliation	CNRS
contact email	fbertile@unistra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD013039

PRIDE project URI

• PRIDE
  1. PXD013039
     1. Label: PRIDE project
     2. Name: Circadian analysis of the mouse cerebellum