PXD013173

PXD013173 is an original dataset announced via ProteomeXchange.

Title	Global Proteomic Analysis of Lysine Succinylation in Zebrafish (Danio rerio)
Description	Lysine succinylation (Ksu) is a novel identified post-translational modification that conserved from prokaryote to eukaryotes. As a kind of acylation, Ksu was reported to have different functions with others acylation at lysine residue. However, recently studies on the Ksu mainly focus on the plants and bacterial, there are still very rare studies in the vertebrate. Therefore, the biological role of succinylation remains largely unknown in mammal. In this study, we performed global Ksu mapping in Danio rerio (zebrafish) using mass spectrometry-based proteomics with enrichment of Ksu peptides by immunoprecipitation technology. As a result, we totally identified 552 Ksu sites in 164 proteins. Compared with our previous studies on lysine acetylation and crotonylation, Ksu plays a major role in a diverse metabolic process, such as carbon metabolism and tricarboxylic acid circle. In addition, we defined 5 new succinylation motifs: (su)KA, (suc)KxxxxA, (su)KxxxxL, (su)KxA, (su)KxV. In conclusion, our result provides proteome-wide database for study of Ksu in zebrafish and our bioinformatics result facilitated the understanding of the Ksu in the role of central metabolism.
HostingRepository	PRIDE
AnnounceDate	2019-11-27
AnnouncementXML	Submission_2019-11-27_05:59:38.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yan Gao
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	succinylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-21 02:22:38	ID requested
1	2019-09-20 08:26:29	announced
⏵ 2	2019-11-27 05:59:40	announced	2019-11-27: Updated project metadata.

Gao Y, Lee H, Kwon OK, Tan M, Kim KT, Lee S, ). J Proteome Res, 18(10):3762-3769(2019) [pubmed]

curator keyword: Biological

submitter keyword: Zebrafish larvae,LC-MS/MS, lysine succinylation

Sangkyu Lee
contact affiliation	Associate Professor College of Pharmacy, Kyungpook National University
contact email	pharm239@gmail.com
lab head
Yan Gao
contact affiliation	Kyungpook National University
contact email	gaoyan0723@hotmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD013173
     1. Label: PRIDE project
     2. Name: Global Proteomic Analysis of Lysine Succinylation in Zebrafish (Danio rerio)