PXD013972
PXD013972 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Glycan Markers of Human Stem Cells Assigned with Beam Search Arrays |
Description | Glycan antigens recognized by monoclonal antibodies have served as stem cell markers. To understand regulation of their biosynthesis and their roles in stem cell behavior precise assignments are required. We have applied state-of-the-art glycan array technologies to compare the glycans bound by five antibodies that recognize carbohydrates on human stem cells. These are: FC10.2, TRA-1-60, TRA-1-81, anti-i and R-10G. Microarray analyses with a panel of sequence-defined glycans corroborate that FC10.2, TRA-1-60, TRA-1-81 recognize the type 1-based backbone sequence, Galβ-3GlcNAcβ-3Galβ-4GlcNAcβ-3Galβ-4GlcNAc, and anti-i, the type 2-analog, Galβ-4GlcNAcβ-3Galβ-4GlcNAcβ-3Galβ-4GlcNAc, and we determine substituents they can accommodate. They differ from R-10G, which requires sulfate. By Beam Search approach, starting with an antigen-positive keratan sulfate polysaccharide, followed by targeted iterative microarray analyses of glycan populations released with keratanases and mass spectrometric monitoring, R-10G is assigned as a unique mono-sulfated type 2 chain, Galβ-4GlcNAc(6S)β-3Galβ-4GlacNAcβ-3Galβ-4GlcNAc. Microarray analyses using newly synthesized glycans corroborate the assignment of this unique determinant raising questions regarding involvement as a ligand in the iPS cell niche. |
HostingRepository | jPOST |
AnnounceDate | 2019-06-18 |
AnnouncementXML | Submission_2022-09-18_03:25:40.482.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yukie Akune |
SpeciesList | scientific name: cellular organisms; NCBI TaxID: 131567; |
ModificationList | unknown modification |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2019-05-22 05:49:09 | ID requested | |
1 | 2019-09-17 03:26:09 | announced | |
2 | 2021-02-04 16:49:04 | announced | 2021-02-04: Updated FTP location. |
⏵ 3 | 2022-09-18 03:25:41 | announced | 2022-09-18: Updated FTP location. |
Publication List
Wu N, Silva LM, Liu Y, Zhang Y, Gao C, Zhang F, Fu L, Peng Y, Linhardt R, Kawasaki T, Mulloy B, Chai W, Feizi T, Glycan Markers of Human Stem Cells Assigned with Beam Search Arrays. Mol Cell Proteomics, 18(10):1981-2002(2019) [pubmed] |
Keyword List
submitter keyword: glycan microarray, human stem cell, antibody R-10G, keratan sulfate, beam search array |
Contact List
Ten Feizi | |
---|---|
lab head | |
Yukie Akune | |
contact affiliation | Imperial College London |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000605/ |