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PXD016813

PXD016813 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleArginine and lysine methylproteomes of Giardia duodenalis
DescriptionMethylation is a common post-translational modification of lysine and arginine in eukaryotic proteins. To date, these methylomes are best characterised in model organisms and higher eukaryotes. Herein, we integrated bioinformatics, proteomics and high-content drug-screening to comprehensively explore protein methylation in the human gastrointestinal parasite and deep-branching eukaryote, Giardia duodenalis. We demonstrate Giardia and other Diplomonadida species lack arginine-methyltransferases and have remodelled RGG/RG motifs preferred by these enzymes. Further, Giardia had no detectable methylarginine in vitro, demonstrating the first eukaryote with no arginine methylome. In contrast, we performed detailed curation of 11 putative lysine-methyltransferases, including highly-diverged SET-domain proteins, and novel annotations demonstrating conserved eEF1a methyllysine. We identified >200 high-confidence methyllysine sites, highlighting methyllysine within coiled-coil features, and for Giardia cytoskeletal regulation. Lastly, using known methylation-inhibitors, we demonstrate inhibition of methylation plays a key role in replication and cyst formation in this parasite.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:50:32.808.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSamantha Emery
SpeciesList scientific name: Giardia lamblia ATCC 50803; NCBI TaxID: 184922;
ModificationListmonomethylated residue; monohydroxylated residue; dimethylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-12-18 01:26:12ID requested
12020-08-07 05:01:23announced
22023-11-14 08:50:33announced2023-11-14: Updated project metadata.
Publication List
Emery-Corbin SJ, Hamey JJ, Ansell BRE, Balan B, Tichkule S, Stroehlein AJ, Cooper C, McInerney BV, Hediyeh-Zadeh S, Vuong D, Crombie A, Lacey E, Davis MJ, Wilkins MR, Bahlo M, Sv, รค, rd SG, Gasser RB, Jex AR, Eukaryote-Conserved Methylarginine Is Absent in Diplomonads and Functionally Compensated in Giardia. Mol Biol Evol, 37(12):3525-3549(2020) [pubmed]
Keyword List
submitter keyword: methylation, arginine methylation, PTM,Giardia, lysine methylation
Contact List
Aaron R Jex
contact affiliationPopulation Health and Immunity Division, Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC, Australia Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Melbourne, VIC, Australia
contact emailjex.a@wehi.edu.au
lab head
Samantha Emery
contact affiliationPopulation Health and Immunity, Walter and Eliza Hall Institute of Medical Research
contact emailemery.s@wehi.edu.au
dataset submitter
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Dataset FTP location
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PRIDE project URI
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