PXD017873

PXD017873 is an original dataset announced via ProteomeXchange.

Title	Structure of Cyanobacterial Phycobilisome Core Revealed by Structural Modeling and Chemical Cross-linking
Description	In cyanobacteria and red algae, the structural basis dictating efficient excitation energy transfer from the phycobilisome (PBS) antenna complex to the reaction centers (RCs) remains unclear. PBS has several peripheral rods and a central core, which binds to the thylakoid membrane, allowing energy coupling with Photosystems II (PSII) and Photosystem I (PSI). Here, we integrated chemical cross-linking mass spectrometry with homology modeling analysis to propose a tri-cylindrical cyanobacterial PBS-core structure. Our model reveals a side view crossover configuration of the two basal cylinders, consolidating the essential roles of the anchoring domains comprised of the ApcE PB-loop and ApcD, which facilitate the energy transfer to PSII and PSI respectively. The uneven bottom surface of the PBS-core contrasts with the flat reducing side of PSII. The extra space between two basal cylinders of the PBS-core and PSII provides increased accessibility of regulatory elements, e.g., orange carotenoid protein, which are required for modulating photochemical activities.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-08_18:06:55.112.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD017873
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Haijun Liu
SpeciesList	scientific name: Synechocystis sp. PCC 6803 substr. GT-S; NCBI TaxID: 1111707;
ModificationList	Ammonia-loss; Phycocyanobilin; Methyl; Formyl; Oxidation; Carbamyl; acetylated residue; deaminated residue; Carbamidomethyl; iodoacetamide derivatized residue; Gln->pyro-Glu; Delta:H(4)C(3); Phospho; Deamidated; Dioxidation; Acetyl; monohydroxylated residue; Trimethyl
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-03-04 02:10:04	ID requested
⏵ 1	2021-09-08 18:06:55	announced

Liu H, Zhang MM, Weisz DA, Cheng M, Pakrasi HB, Blankenship RE, Structure of cyanobacterial phycobilisome core revealed by structural modeling and chemical cross-linking. Sci Adv, 7(2):(2021) [pubmed]

submitter keyword: Phycobilisome, Phycobilisome core structure, Protein structure prediction, I-TASSER, Mass Spectrometry, Protein Cross-linking, Photosynthesis

Haijun Liu
contact affiliation	Department of Chemistry Washington University in St. Louis
contact email	liuhaijun@wustl.edu
lab head
Haijun Liu
contact affiliation	Washington University in St. Louis
contact email	haijunmariner@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD017873
     1. Label: PRIDE project
     2. Name: Structure of Cyanobacterial Phycobilisome Core Revealed by Structural Modeling and Chemical Cross-linking