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PXD020344

PXD020344 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleN-glycoproteins have a major role in MGL binding to colorectal cancer cell lines
DescriptionColorectal cancer (CRC) is the second leading cause of cancer death worldwide due in part to a high proportion of patients diagnosed at advanced stages of the disease. For this reason, many efforts have been made towards new approaches for early detection and prognosis. Cancer-associated aberrant glycosylation, especially the Tn and STn antigens, can be detected using the macrophage galactose-type C-type lectin (MGL/CLEC10A/CD301), which has been shown to be a promising tool for CRC prognosis. We had recently identified the major MGL binding glycoproteins in two high MGL binding CRC cells lines, HCT116 and HT29. We investigated here the impact of N-linked and O-linked glycans carried by these proteins for the binding to MGL. In addition, we performed quantitative proteomics to study the major differences in proteins involved in glycosylation in these cells. Our results showed that N-glycans have a significant, previously underestimated, importance in MGL binding to CRC cell lines.
HostingRepositoryPRIDE
AnnounceDate2020-08-10
AnnouncementXMLSubmission_2020-08-09_22:59:41.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYassene Mohammed
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-07-13 17:44:12ID requested
12020-08-09 22:59:42announced
Publication List
Pirro M, Mohammed Y, van Vliet SJ, Rombouts Y, Sciacca A, de Ru AH, Janssen GMC, Tjokrodirijo RTN, Wuhrer M, van Veelen PA, Hensbergen PJ, -Glycoproteins Have a Major Role in MGL Binding to Colorectal Cancer Cell Lines: Associations with Overall Proteome Diversity. Int J Mol Sci, 21(15):(2020) [pubmed]
Keyword List
submitter keyword: Glycoproteomics
TMT labelling
C-type lectin
Colorectal cancer
LacdiNAc
Tn antigen
Contact List
Paul J. Hensbergen
contact affiliationCenter for Proteomics and Metabolomics Leiden University Medical Center
contact emailP.J.Hensbergen@lumc.nl
lab head
Yassene Mohammed
contact affiliationLUMC/UVIC
contact emaily.mohammed@lumc.nl
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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