PXD031451

PXD031451 is an original dataset announced via ProteomeXchange.

Title	Immunopeptidomics-based design of highly effective mRNA vaccine formulations against Listeria monocytogenes
Description	Listeria monocytogenes is a foodborne intracellular bacterial pathogen leading to human listeriosis. Despite a high mortality rate and increasing antibiotic resistance no clinically approved vaccine against Listeria is available. To identify antigens for this bacterial pathogen that can be encoded in mRNA vaccine formulations, we screened for Listeria epitopes presented on the surface of infected human cell lines by mass spectrometry-based immunopeptidomics. In between more than 15,000 human self-peptides, we detected 68 Listeria epitopes from 42 different bacterial proteins, including several known antigens. Peptide epitopes presented on different cell lines were often derived from the same bacterial surface proteins, classifying these antigens as potential vaccine candidates. Encoding these highly presented antigens in lipid nanoparticle mRNA vaccine formulations resulted in high levels of protection in vaccination challenge experiments in mice. Our results pave the way for the development of a clinical mRNA vaccine against Listeria and demonstrate the power of immunopeptidomics for next-generation bacterial vaccine development.
HostingRepository	PRIDE
AnnounceDate	2022-09-26
AnnouncementXML	Submission_2022-09-26_06:39:50.062.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD031451
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Rupert Mayer
SpeciesList	scientific name: Listeria monocytogenes EGD; NCBI TaxID: 1334565; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Q Exactive HF; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-04 15:42:21	ID requested
⏵ 1	2022-09-26 06:39:50	announced

Dataset with its publication pending

ProteomeXchange project tag: Human Immuno-Peptidome Project (HUPO-HIPP) (B/D-HPP)

submitter keyword: Q Exactive HF, Fusion Lumos, immunopeptidomics, antigen discovery, epitope discovery, Listeria monocytogenes, bacterial infection, vaccine development, HeLa, HCT-116, label-free, TMT

Francis Impens
contact affiliation	VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium. Department of Biomolecular Medicine, Ghent University, Ghent, Belgium. VIB Proteomics Core, VIB, Ghent, Belgium.
contact email	francis.impens@vib-ugent.be
lab head
Rupert Mayer
contact affiliation	Department of Analytical Chemistry, Faculty of Chemistry, University of Vienna
contact email	rupert.mayer@vib-ugent.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031451
     1. Label: PRIDE project
     2. Name: Immunopeptidomics-based design of highly effective mRNA vaccine formulations against Listeria monocytogenes