PXD034353 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Passive elution of proteoforms from polyacrylamide gels combined with anion exchange purification for in-depth top-down analysis of the human low molecular weight proteome. |
Description | We developed a novel sample preparation method by combining Passively Eluting Proteins from Polyacrylamide gels as Intact species for MS (PEPPI-MS) fractionation with Anion-Exchange disc-assisted Sequential sample Preparation (AnExSP) purification, and evaluated its performance in TDP analysis by comparing it with a conventional PEPPI workflow using Methanol-Chloroform-Water (MCW) precipitation. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:57:02.853.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Andreas Tholey |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-06-06 02:19:06 | ID requested | |
1 | 2022-09-26 02:41:11 | announced | |
⏵ 2 | 2023-11-14 08:57:03 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: SDS-PAGE,Top-down, PEPPI, Anion Exchange, StageTip |
Contact List
Andreas Tholey |
contact affiliation | Systematic Proteome Research & Bioanalytics, Institute for Experimental Medicine, Christian-Albrechts-Universität zu Kiel, 24105 Kiel, Germany |
contact email | a.tholey@iem.uni-kiel.de |
lab head | |
Andreas Tholey |
contact affiliation | Systematic Proteome Research & Bioanalytics, University of Kiel |
contact email | a.tholey@iem.uni-kiel.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD034353 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034353
- Label: PRIDE project
- Name: Passive elution of proteoforms from polyacrylamide gels combined with anion exchange purification for in-depth top-down analysis of the human low molecular weight proteome.