PXD034435

PXD034435 is an original dataset announced via ProteomeXchange.

Title	Gigartina pilot scale protein extract BUP
Description	Seaweeds are attracting substantial interest as novel sources of sustainable food protein, as they are established sources of industrial hydrocolloids and have reasonable protein content. In this study we investigate the protein composition and quality of a seaweed protein extract (SPE) from Gigartina radula obtained as a side-stream from industrial carrageenan production. The SPE displayed low (<2%), but pH-dependent, aqueous solubility due to the harsh (heat and extreme pH) employed during extraction. Solubility was improved using buffer and detergent to facilitate proteomic characterization by quantitative LC-MS/MS. Proteomics revealed that the SPE was dominated by proteins related to light harvest and particularly rich in phycobiliproteins (44% relative molar abundance), where phycoerythrin was the most abundant (28%). Based on subcellular localization of identified proteins, the extraction method was evaluated as good for release of cellular protein. The SPE was found to be rich in essential amino acids (36-41%) and particularly in branched chain amino acids (22-24%) and thereby a potential source of nutritional food protein. Using bioinformatic prediction and structural modelling, we found that abundant proteins in the SPE contained high potential, novel emulsifier peptides with amphiphilic properties required to stabilize an oil/water interface. Based on this study, Gigartina could serve as a good candidate for extraction of sustainable and nutritious food protein and possibly be further processed to obtain a hydrolysate with good emulsifying properties for use in foods.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:17:29.379.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD034435
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Simon Gregersen Echers
SpeciesList	scientific name: Solanum tuberosum (Potato); NCBI TaxID: 4113;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-09 14:27:40	ID requested
1	2022-09-26 02:03:13	announced
⏵ 2	2023-11-14 07:17:29	announced	2023-11-14: Updated project metadata.

10.6019/PXD034435;

submitter keyword: Gigartina, Rhodophyta, Alternative Food Protein, Seaweed

Michael Toft Overgaard
contact affiliation	Department of Chemistry and Bioscience Aalborg University Denmark
contact email	mto@bio.aau.dk
lab head
Simon Gregersen Echers
contact affiliation	Department of Chemistry & Bioscience Aalborg University
contact email	sgr@bio.aau.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034435
     1. Label: PRIDE project
     2. Name: Gigartina pilot scale protein extract BUP