PXD039959

PXD039959 is an original dataset announced via ProteomeXchange.

Title	TBC1D2B undergoes phase separation and mediates autophagy initiation
Description	Small ubiquitin-like modifiers from the ATG8 family regulate autophagy initiation and progression in mammalian cells. Their interaction with LC3-interacting region (LIR) containing proteins promotes cargo sequestration, phagophore assembly, or even fusion between autophagosomes and lysosomes. Previously, we have shown that RabGAP proteins from the TBC family directly bind to LC3/GABARAP proteins. In the present study, we focus on the function of TBC1D2B. We show that TBC1D2B contains a functional canonical LIR motif and acts at an early stage of autophagy by binding to both LC3/GABARAP proteins and ATG5 conjugation machinery. Subsequently, TBC1D2B is degraded by autophagy. TBC1D2B condensates into liquid droplets upon autophagy induction. Our study suggests that phase separation is an underlying mechanism of TBC1D2B-dependent autophagy induction, which serves as an assembly platform for further cargo sequestration.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:04:12.008.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marina Hoffmann
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-08 06:38:50	ID requested
1	2023-09-28 11:03:07	announced
2	2023-11-14 08:53:39	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:04:12	announced	2024-10-22: Updated project metadata.

10.1002/JCB.30481;

submitter keyword: TBC1D2B,autophagy, phagophore formation, ATG8-related proteins, liquid-liquid phase separation, GTPase-activating protein, autophagy conjugation complex

Ivan Dikic
contact affiliation	Institute of Biochemistry II, Faculty of Medicine, Goethe University Frankfurt, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany. Buchmann Institute for Molecular Life Sciences, Goethe University Frankfurt, Max-von-Laue Straße 15, 60438 Frankfurt am Main, Germany. Fraunhofer Institute of Translational Medicine and Pharmacology, Carl-von-Noorden-Platz 9, 60596 Frankfurt am Main, Germany
contact email	dikic@biochem2.uni-frankfurt.de
lab head
Marina Hoffmann
contact affiliation	institute of biochemistry 2 (IBC2)
contact email	hoffmann@med.uni-frankfurt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD039959

PRIDE project URI

• PRIDE
  1. PXD039959
     1. Label: PRIDE project
     2. Name: TBC1D2B undergoes phase separation and mediates autophagy initiation