PXD042082

PXD042082 is an original dataset announced via ProteomeXchange.

Title	Immortalized mouse caput epididymal (mECap18) cell line recapitulates in vivo environment
Description	Between the testes and the vas deferens lies the epididymis, a highly convoluted tubule whose unique environmental characteristics are crucial for the functional maturation of spermatozoa. Within the epididymal epithelium, the secretory system releases small non-coding RNA molecules (sncRNAs) and proteins housed within extracellular vesicles (epididymosomes) that are destined for delivery to recipient sperm cells which play key roles in fertility success and act as major conduits of epigenetic information delivered to the oocyte. The epithelial cells of the epididymis have proven to be highly sensitive to environmental stressors which can influence the sperm epigenome. Utilizing a label-free mass spectrometry proteomic approach we sought to characterize an immortalized mouse caput epididymal epithelial cell line (mECap18) and sequenced >5,100 proteins. When compared to a previous in-vivo mouse caput epithelial proteomic profile, a significant overlap (>75%) and proportionally similar patterns of protein classification were observed. Furthermore, key pathways associated with protein synthesis (e.g. EIF2 signaling) and cellular protection in the male reproductive tract (e.g. sirtuin signaling) were enriched in both proteomes. Leveraging this comparison supports mECap18 cells as a promising in-vitro model for recapitulating the in-vivo environment, providing a platform for testing therapeutic invention.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:04:12.731.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD042082
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Skerrett-Byrne
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-09 01:18:07	ID requested
1	2023-09-28 20:18:26	announced
2	2023-11-14 09:05:39	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:04:13	announced	2024-10-22: Updated project metadata.

Mulhall JE, Trigg NA, Bernstein IR, Anderson AL, Murray HC, Sipil, ä P, Lord T, Schjenken JE, Nixon B, Skerrett-Byrne DA, Immortalized mouse caput epididymal epithelial (mECap18) cell line recapitulates the in-vivo environment. Proteomics, 24(7):e2300253(2024) [pubmed]

10.1002/pmic.202300253;

10.6019/PXD042082;

submitter keyword: fertility,epididymis, male reproduction, sperm maturation, proteomics.

David Skerrett-Byrne
contact affiliation	Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, College of Engineering, Science and Environment, The University of Newcastle, NSW, Australia. Infertility and Reproduction Research Program, Hunter Medical Research Institute, NSW, Australia.
contact email	David.Skerrett-Byrne@newcastle.edu.au
lab head
David Skerrett-Byrne
contact affiliation	Helmholtz Zentrum München
contact email	David.Skerrett-Byrne@newcastle.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042082
     1. Label: PRIDE project
     2. Name: Immortalized mouse caput epididymal (mECap18) cell line recapitulates in vivo environment