PXD045596

PXD045596 is an original dataset announced via ProteomeXchange.

Title	Plasticity of the leaf lipidome and proteome and in response to pathogen infection and heat stress
Description	Plants must cope with a variety of different stressors during their life cycle and their adaptations to these environmental influences involves all cellular organelles. Among them, comparatively little is known about the contribution of cytosolic lipid droplets (LDs) and their core of neutral lipids and associated surface proteins to the rewiring of cellular processes in response to stress. Here, we analyzed the changes that occur in the lipidome and proteome of Arabidopsis leaves after pathogen infections with Botrytis cinerea and Pseudomonas syringae, and heat stress. Analyses were carried out in wild-type plants and the oil-rich double mutant tgd1-1 sdp1-4 that allowed an allied study of the LD proteome in stressed leaves. Using liquid chromatography-tandem mass spectrometry-based methods, we show that a hyperaccumulation of the primary LD core component triacylglycerol is a general response to stress and that acyl chains are remodeled during cellular adaptation. Likewise, comparative analysis of the LD protein composition in stress-treated leaves highlighted the remodeling of the LD proteome as part of the general stress response. We further identified two new LD-associated proteins whose localization to LDs in leaves was confirmed by confocal microscopy of fluorescent protein fusions. Taken together, these and other results highlight LDs as dynamic contributors to the cellular adaptation processes that underlie how plants respond to environmental stress.
HostingRepository	PRIDE
AnnounceDate	2025-03-21
AnnouncementXML	Submission_2025-03-21_07:34:02.803.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Till Ischebeck
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF; LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-22 07:00:21	ID requested
⏵ 1	2025-03-21 07:34:03	announced

10.1093/PLPHYS/KIAE274;

submitter keyword: Lipid droplets, Botrytis cinerea,Arabidopsis, Pseudomonas syringiae, Heat stress

Till Ischebeck
contact affiliation	University of Münster Institute of Plant Biology and Biotechnology
contact email	till.ischebeck@uni-muenster.de
lab head
Till Ischebeck
contact affiliation	University of Münster
contact email	tischeb@gwdg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045596
     1. Label: PRIDE project
     2. Name: Plasticity of the leaf lipidome and proteome and in response to pathogen infection and heat stress