PXD049123

PXD049123 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomics of Hela cells stimulated with EGF in the presence of protein phosphatase inhibitors
Description	We profiles the effects of six protein phosphatase inhibitors on EGF-dependent phosphoproteome dynamics. The cells were treated with 20 nM EGF for 8 or 20 min with or without 15 min pre-treatment of one of the phosphatase inhibitors (100 nM or 1 M; Raphin1 acetate, sanguinarine chloride, KY-226, SHP099, NSC95397, or BCI). We further focused on sanguinarine chloride, and Hela cells were treated with 20 nM EGF for 3, 8, or 20min with or without 15 min pre-treatment of sanguinarine (5, 50, or 500 nM). We also examined combination of sanguinarine chloride (500 nM) and BIRB796 (500 nM), a p38-kinase inhibitor.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:37:43.492.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Akihiro Eguchi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-02 09:19:47	ID requested
1	2024-04-29 22:28:17	announced
⏵ 2	2024-10-22 06:37:43	announced	2024-10-22: Updated project metadata.

10.1038/S41598-024-58619-1;

submitter keyword: Human, Phosphoproteomics, Protein Phosphatases, LC-MS/MS

Jesper. V. Olsen
contact affiliation	Proteomics Program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
contact email	jesper.olsen@cpr.ku.dk
lab head
Akihiro Eguchi
contact affiliation	Novo Nordisk Foundation Center for Protein Research, University of Copenhagen
contact email	akihiro.eguchi@cpr.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD049123

PRIDE project URI

• PRIDE
  1. PXD049123
     1. Label: PRIDE project
     2. Name: Phosphoproteomics of Hela cells stimulated with EGF in the presence of protein phosphatase inhibitors