PXD061496

PXD061496 is an original dataset announced via ProteomeXchange.

Title	Snf2 interactome in either WT or set1∆ strains
Description	Set1 is the catalytic subunit of an evolutionarily conserved complex, SET1C or COMPASS, which methylates histone H3 at lysine 4 and serves as a scaffold for the association of seven tightly bound polypeptides: Swd1, Swd2, Swd3, Bre2, Sdc1, Spp1, and Shg1. Our results reveal that Set1 interacts with several importins as well as RGG motif-containing proteins, providing new insights into the mechanisms by which Set1 moves between cytoplasmic and nuclear compartments. We uncover that SET1C likely cooperates with other chromatin regulators, particularly via the Snf2 component of the SWI/SNF complex. Here to further investigate Snf2 methylation by Set1C in vivo, we purified Snf2-GFP and its subunits with GFP nanobodies in the presence or absence of Set1. The protein composition of the complex was determined by mass spectrometry in the presence of absence of Set1.
HostingRepository	PRIDE
AnnounceDate	2026-09-21
AnnouncementXML	Submission_2026-09-20_19:23:59.717.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luc Camoin
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-05 02:10:54	ID requested
⏵ 1	2026-09-20 19:24:00	announced

10.7554/elife.109886;

Luciano P, Park K, Audebert S, Camoin L, Ni, ñ, o CA, Park DK, Maudlin IE, Dubarry M, Lee L, Oeffinger M, Beggs JD, Kim YH, Kim J, Dichtl B, Geli V, SET1C expands its functional landscape and identifies candidate regulatory connections. Elife, 15():(2026) [pubmed]

submitter keyword: Snf2, LC-MS-MS, Saccharomyces cerevisiae

Camoin Luc - Audebert Stephane
contact affiliation	Marseille Protéomique Centre de Recherche en Cancérologie de Marseille Institut Paoli Calmettes - Inserm U1068 - CNRS UMR7258 - Aix Marseille Université UM105 27, Bd Leï Roure, BP30059, 13273 Marseille Cedex 9, France
contact email	luc.camoin@inserm.fr
lab head
Luc Camoin
contact affiliation	Life Sciences
contact email	luc.camoin@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD061496

PRIDE project URI

• PRIDE
  1. PXD061496
     1. Label: PRIDE project
     2. Name: Snf2 interactome in either WT or set1∆ strains