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DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2014-08-08
  • AnnouncementXML: Submission_2014-08-08_00:31:23.xml
  • DigitalObjectIdentifier: http://dx.doi.org/10.6019/PXD000461
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Supported dataset by repository
  • PrimarySubmitter: Kazunobu Saito
  • Title: Identification of telomere-binding proteins by enChIP-MS
  • Description: We identified the telomere associated molecules by engineered DNA-binding molecule-mediated chromatin immunoprecipitaion (enChIP). Purified proteins were analyzed by GeLC-MS/MS. Tandem mass spectra were extracted by Proteome Discoverer 1.2. Database searchs were submitted to an in-house Mascot server version 2.4.1. Mascot was set up to search the Swissprot database assuming the digestion enzyme as trypsin. Carbamidomethylation of cysteine was set as a fixed modification. Oxidized methionine, acetylation of N terminus and conversion of glutamine to Pyro-Glu at N terminus were set as variable modifications. The precursor mass tolerances were 10 ppm and the tolerance of the MS/MS ions was 0.8 Da. In Scaffold 3.4.5, database search results were grouped according to gel bands.
  • SpeciesList: scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
  • ModificationList: monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deaminated residue
  • Instrument: LTQ Orbitrap Velos; instrument model: LTQ Orbitrap velos

Dataset History

VersionDatetimeStatusChangeLog Entry
02013-09-12 04:04:20ID requested
12013-11-12 03:36:17announced
12013-11-12 04:31:37announced
22014-07-28 00:48:10announcedUpdated project metadata.
32014-08-08 00:31:23announcedUpdated project metadata.

Publication List

  1. Fujita T, Asano Y, Ohtsuka J, Takada Y, Saito K, Ohki R, Fujii H, Identification of telomere-associated molecules by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP). Sci Rep, 3():3171(2013) [pubmed]

Keyword List

  1. curator keyword: Biomedical, Biological
  2. submitter keyword: mouse, Telomere binding protein, LC-MS/MS

Contact List

    Kazunobu Saito
    • contact affiliation: Research Institute for Microbial Diseases
    • contact email: kazusa@biken.osaka-u.ac.jp
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List
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