PXD000461-3

PXD000461 is an original dataset announced via ProteomeXchange.

Title	Identification of telomere-binding proteins by enChIP-MS
Description	We identified the telomere associated molecules by engineered DNA-binding molecule-mediated chromatin immunoprecipitaion (enChIP). Purified proteins were analyzed by GeLC-MS/MS. Tandem mass spectra were extracted by Proteome Discoverer 1.2. Database searchs were submitted to an in-house Mascot server version 2.4.1. Mascot was set up to search the Swissprot database assuming the digestion enzyme as trypsin. Carbamidomethylation of cysteine was set as a fixed modification. Oxidized methionine, acetylation of N terminus and conversion of glutamine to Pyro-Glu at N terminus were set as variable modifications. The precursor mass tolerances were 10 ppm and the tolerance of the MS/MS ions was 0.8 Da. In Scaffold 3.4.5, database search results were grouped according to gel bands.
HostingRepository	PRIDE
AnnounceDate	2014-08-08
AnnouncementXML	Submission_2014-08-08_00:31:23.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000461
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kazunobu Saito
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deaminated residue
Instrument	LTQ Orbitrap Velos; instrument model: LTQ Orbitrap velos

Revision	Datetime	Status	ChangeLog Entry
0	2013-09-12 04:04:20	ID requested
1	2013-11-12 03:36:17	announced
1	2013-11-12 04:31:37	announced
2	2014-07-28 00:48:10	announced	Updated project metadata.
⏵ 3	2014-08-08 00:31:23	announced	Updated project metadata.
4	2024-10-07 09:08:25	announced	2024-10-07: Updated project metadata.

Fujita T, Asano Y, Ohtsuka J, Takada Y, Saito K, Ohki R, Fujii H, Identification of telomere-associated molecules by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP). Sci Rep, 3():3171(2013) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: mouse, Telomere binding protein, LC-MS/MS

Kazunobu Saito
contact affiliation	Research Institute for Microbial Diseases
contact email	kazusa@biken.osaka-u.ac.jp
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/11/PXD000461

PRIDE project URI

• PRIDE
  1. PXD000461
     1. Label: PRIDE project
     2. Name: Identification of telomere-binding proteins by enChIP-MS