PXD004940-2

PXD004940 is an original dataset announced via ProteomeXchange.

Title	Performance of the Orbitrap Fusion Lumos Tribrid in single-shot analyses of human samples
Description	One of the major additions in mass spectrometry technology has been the irruption of the Orbitrap mass analyzer, which has boosted the proteomics analyses of biological complex samples since its introduction. Here we assessed the performance of the new generation Orbitrap Fusion Lumos Tribrid mass spectrometer in the identification and quantitation of peptides and phosphopeptides in single-shot analyses of human whole cell lysates using robust data-dependent acquisition methods. Our study explored the capabilities of the instrument for (phospho-)peptide identification and quantitation using different gradient lengths, different sample amounts, and different combinations of peptide fragmentation and mass analysers acquisition methods. Moreover, the acquisition of the same complex sample with different acquisition methods resulted in the generation of a dataset to be used as a reference for further analyses, and a starting point for future optimizations in particular applications.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:32:37.074.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eduard Sabidó
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-09-12 03:47:29	ID requested
1	2017-03-09 02:24:04	announced
⏵ 2	2024-10-22 04:32:41	announced	2024-10-22: Updated project metadata.

Espadas G, Borr, à, s E, Chiva C, Sabid, ó E, Evaluation of different peptide fragmentation types and mass analyzers in data-dependent methods using an Orbitrap Fusion Lumos Tribrid mass spectrometer. Proteomics, 17(9):(2017) [pubmed]

10.1002/pmic.201600416;

curator keyword: Technical

submitter keyword: Protein quantitation,Orbitrap Fusion Lumos, mass spectrometry, phosphorylation, protein identification

Eduard Sabidó
contact affiliation	1. Proteomics Unit, Centre de Regulació Genòmica, 08003 Barcelona, Spain 2. Proteomics Unit, Universitat de Pompeu Fabra, 08003 Barcelona, Spain
contact email	eduard.sabido@crg.cat
lab head
Eduard Sabidó
contact affiliation	Centre de Regulació Genòmica
contact email	eduard.sabido@crg.cat
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/03/PXD004940

PRIDE project URI

• PRIDE
  1. PXD004940
     1. Label: PRIDE project
     2. Name: Performance of the Orbitrap Fusion Lumos Tribrid in single-shot analyses of human samples