PXD005150-4

PXD005150 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of Kveim reagent identifies targets of cellular immunity in sarcoidosis
Description	Kveim-reagent (Kv) skin testing was a historical method of diagnosing sarcoidosis. Intradermal injection of treated sarcoidosis spleen tissue resulted in a granuloma response at injection site by 4-6 weeks. Previous work indicates proteins as the possible trigger of this reaction. We aimed to identify Kv-specific proteins and characterise the ex vivo response of Peripheral Blood Mononuclear Cells (PBMCs) from sarcoidosis, tuberculosis and healthy control patients when stimulated with both Kv and selected Kv-specific proteins.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_05:22:33.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005150
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Robert Parker
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamyl; Acetyl; Carbamidomethyl
Instrument	6520 Quadrupole Time-of-Flight LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-14 06:17:57	ID requested
1	2017-01-31 02:07:29	announced
2	2017-02-16 06:48:55	announced	Updated project metadata.
3	2017-10-24 05:20:19	announced	Updated project metadata.
⏵ 4	2017-10-24 05:22:34	announced	Updated project metadata.

Eberhardt C, Thillai M, Parker R, Siddiqui N, Potiphar L, Goldin R, Timms JF, Wells AU, Kon OM, Wickremasinghe M, Mitchell D, Weeks ME, Lalvani A, Proteomic Analysis of Kveim Reagent Identifies Targets of Cellular Immunity in Sarcoidosis. PLoS One, 12(1):e0170285(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Sarcoidosis, Immunology, Proteomics, Interstitial Lung Diseases

Ajit Lalvani
contact affiliation	Imperial College London
contact email	a.lalvani@imperial.ac.uk
lab head
Robert Parker
contact affiliation	Centre for High Throughput Biology
contact email	robert.rparker@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/01/PXD005150

PRIDE project URI

• PRIDE
  1. PXD005150
     1. Label: PRIDE project
     2. Name: Proteomic analysis of Kveim reagent identifies targets of cellular immunity in sarcoidosis