PXD006074-1

PXD006074 is an original dataset announced via ProteomeXchange.

Title	ZBTB48 is both a vertebrate telomere-binding protein and a transcriptional activator
Description	Telomeres constitute the ends of linear chromosomes and together with the shelterin complex form a structure essential for genome maintenance and stability. In addition to the constitutive binding of the shelterin complex, other direct, yet more transient interactions are mediated by the CST complex and HOT1, while subtelomeric variant repeats are recognized by NR2C/F transcription factors. Recently, the Kruppel-like zinc finger protein ZBTB48 has been described as a novel telomere-associated factor in the vertebrate lineage. Here, we show that ZBTB48 binds directly both to telomeric as well as to subtelomeric variant repeat sequences. ZBTB48 is found at telomeres of human cancer cells regardless of the mode of telomere maintenance and it acts as a negative regulator of telomere length. In addition to its telomeric function, we demonstrate through a combination of RNAseq, ChIPseq and expression proteomics experiments that ZBTB48 acts as a transcriptional activator on a small set of target genes, including mitochondrial fission process 1 (MTFP1). This discovery places ZBTB48 at the interface of telomere length regulation, transcriptional control and mitochondrial metabolism.
HostingRepository	PRIDE
AnnounceDate	2017-05-11
AnnouncementXML	Submission_2017-05-11_00:23:01.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	F Butter
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-13 02:14:23	ID requested
⏵ 1	2017-05-11 00:23:02	announced
2	2017-05-16 09:18:59	announced	Updated publication reference for PubMed record(s): 28500257.
3	2024-10-22 04:08:53	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: ZBTB48

HeLa

U2OS

knock-out

Falk Butter
contact affiliation	Institute of Molecular Biology Ackermannweg 4 55128 Mainz
contact email	f.butter@imb-mainz.de
lab head
F Butter
contact affiliation	Dept. of Proteomics and Signal Transduction
contact email	f.butter@imb-mainz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD006074

PRIDE project URI

• PRIDE
  1. PXD006074
     1. Label: PRIDE project
     2. Name: ZBTB48 is both a vertebrate telomere-binding protein and a transcriptional activator