PXD008680-3

PXD008680 is an original dataset announced via ProteomeXchange.

Title	U2OS Adhesion Isolations +/- CytochalasinD and Neomycin
Description	U2OS cells preferentially utilise integrin alphaV beta5 in adhesion complexes when grown under standard cell culture conditions for 3 days. AlphaV beta5 can be found in both classical 'Focal' adhesions and in novel 'Reticular' adhesions that do not associate with F-actin or other known adhesion protein components. In order to identify components of reticular adhesions, cells were treated with cytochalasinD in order to disrupt F-actin and promote loss of focal adhesions. Remaining reticular adhesion complexes were stabilised with DTBP crosslinking reagent before adhesion complexes were isolated. Preliminary data suggested that depletion of PIP2 by Neomycin treatment would lead to disruption of reticular adhesions preferentially over focal adhesions and so this manipulation was included in these experiments.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:07:07.090.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthew Jones
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-01-15 03:39:40	ID requested
1	2019-06-03 05:59:33	announced
2	2019-06-10 08:49:33	announced	Updated publication reference for PubMed record(s): 30361699.
⏵ 3	2024-10-22 04:07:07	announced	2024-10-22: Updated project metadata.

10.1038/s41556-018-0220-2;

Lock JG, Jones MC, Askari JA, Gong X, Oddone A, Olofsson H, G, ö, ransson S, Lakadamyali M, Humphries MJ, Str, ö, mblad S, Reticular adhesions are a distinct class of cell-matrix adhesions that mediate attachment during mitosis. Nat Cell Biol, 20(11):1290-1302(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Human, CytoD, Reticular, U2OS, Proteomics

Martin J Humphries
contact affiliation	Professor of Biochemistry, Faculty of Biology, Medicine & Health, Wellcome Trust Centre for Cell-Matrix Research, University of Manchester, United Kingdom
contact email	martin.humphries@manchester.ac.uk
lab head
Matthew Jones
contact affiliation	University of Manchester
contact email	matthew.jones-4@manchester.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD008680

PRIDE project URI

• PRIDE
  1. PXD008680
     1. Label: PRIDE project
     2. Name: U2OS Adhesion Isolations +/- CytochalasinD and Neomycin