PXD009237-1

PXD009237 is an original dataset announced via ProteomeXchange.

Title	Human protein phosphorylation analysis
Description	Mycobacterium tuberculosis (Mtb) protein kinase G (PknG) is a critical protein which can inhibit the maturation of phagosome and its fusion with the lysosome, providing Mtb’s latency and survival in the host. However, its signaling pathways and functions in the human body are still unclear and needed to be further studied. In our research, we used the human protein microarray to study the proteins interacting with PknG and have found a list of proteins that are potentially related to PknG in the host, which may contribute to the infection of Mtb. Among these positive hits, we found human protein CypA has strong interaction with PknG. We further validated the interaction between PknG and CypA in vitro and in vivo and investigated its role in the infection of macrophage cells. Finally, we found that PknG can significantly down-regulate protein expression level of CypA through phosphorylation in site of T107 as well as its induction of inflammatory response through NF-κB and ERK1/2 pathways. Meanwhile, the survival of Mycobacterium smegmetis (Msm) in the macrophages with overexpressing PknG is also improved with lessen of cytokines expression. Our results provided a resourceful research base of PknG in regards to various target human proteins and offered an insightful understanding of PknG’s function in the host.
HostingRepository	PRIDE
AnnounceDate	2018-10-10
AnnouncementXML	Submission_2018-10-10_07:42:51.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD009237
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Fanlin Wu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Phospho; Deamidated; Oxidation; Carbamidomethyl
Instrument	ultraflex

Revision	Datetime	Status	ChangeLog Entry
0	2018-03-16 07:41:07	ID requested
⏵ 1	2018-10-10 07:42:52	announced
2	2024-10-22 04:44:04	announced	2024-10-22: Updated project metadata.

Wu FL, Liu Y, Zhang HN, Jiang HW, Cheng L, Guo SJ, Deng JY, Bi LJ, Zhang XE, Gao HF, Tao SC, Global Profiling of PknG Interactions Using a Human Proteome Microarray Reveals Novel Connections with CypA. Proteomics, 18(23):e1800265(2018) [pubmed]

curator keyword: Biological

submitter keyword: human, phosphorylation

Sheng-Ce Tao
contact affiliation	Ph.D., Professor, Vice Dean
contact email	taosc@sjtu.edu.cn
lab head
Fanlin Wu
contact affiliation	Shanghai Jiao Tong University
contact email	wufanlin1990@126.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD009237

PRIDE project URI

• PRIDE
  1. PXD009237
     1. Label: PRIDE project
     2. Name: Human protein phosphorylation analysis