PXD009799

PXD009799 is an original dataset announced via ProteomeXchange.

Title	ICAP2 Immunoprecipitation results
Description	The mitochondrial ATP synthase is a macromolecular motor that uses the proton gradient to generate ATP. Proper ATP synthase function requires a stator linking the catalytic and rotary portions of the complex. However, sequence-based searches fail to identify genes encoding stator subunits in apicomplexan parasites like Toxoplasma gondii or the related organisms that cause malaria. Here, we identify 11 previously unknown subunits from the Toxoplasma ATP synthase, which lack homologs outside the phylum. Hidden Markov modeling suggests that two of them—ICAP2 and ICAP18—share distant homology with mammalian stator subunits. Our mass spcetrometry analysis indicates that both proteins form part of the ATP synthase complex.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:45:16.240.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD009799
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Diego Huet
SpeciesList	scientific name: Toxoplasma gondii ME49; NCBI TaxID: 508771;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-16 06:00:17	ID requested
1	2018-08-22 04:15:26	announced
⏵ 2	2024-10-22 04:45:16	announced	2024-10-22: Updated project metadata.

10.6019/PXD009799;

curator keyword: Biological

submitter keyword: Toxoplasma, ATP synthase, mitochondria

Sebastian Lourido
contact affiliation	Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts USA
contact email	lourido@wi.mit.edu
lab head
Diego Huet
contact affiliation	Whitehead Institute
contact email	dhuet@wi.mit.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009799
     1. Label: PRIDE project
     2. Name: ICAP2 Immunoprecipitation results