<<< Full experiment listing


PXD009799 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleICAP2 Immunoprecipitation results
DescriptionThe mitochondrial ATP synthase is a macromolecular motor that uses the proton gradient to generate ATP. Proper ATP synthase function requires a stator linking the catalytic and rotary portions of the complex. However, sequence-based searches fail to identify genes encoding stator subunits in apicomplexan parasites like Toxoplasma gondii or the related organisms that cause malaria. Here, we identify 11 previously unknown subunits from the Toxoplasma ATP synthase, which lack homologs outside the phylum. Hidden Markov modeling suggests that two of them—ICAP2 and ICAP18—share distant homology with mammalian stator subunits. Our mass spcetrometry analysis indicates that both proteins form part of the ATP synthase complex.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterDiego Huet
SpeciesList scientific name: Toxoplasma gondii ME49; NCBI TaxID: 508771;
ModificationListOxidation; Carbamidomethyl
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-05-16 06:00:17ID requested
12018-08-22 04:15:26announced
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological
submitter keyword: Toxoplasma, ATP synthase, mitochondria
Contact List
Sebastian Lourido
contact affiliationWhitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts USA
contact emaillourido@wi.mit.edu
lab head
Diego Huet
contact affiliationWhitehead Institute
contact emaildhuet@wi.mit.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD009799
PRIDE project URI
Repository Record List
[ + ]