PXD011278-1

PXD011278 is an original dataset announced via ProteomeXchange.

Title	Lactobacillus Membrane Vesicles
Description	Bacterial membrane vesicles have been implicated in a broad range of functions in microbial communities from pathogenesis to gene transfer. Though first thought to be a phenomenon associated with Gram-negative bacteria, vesicle production in Staphylococcus aureus, Lactobacillus plantarum, and other Gram-positives has recently been described. Here we characterize MVs from three different Lactobacillus species (L. acidophilus, L. casei, and L. reuteri), determining that the size and protein composition of Lactobacillus-derived MVs have both similarities and differences with those produced by Gram-negative bacteria. Using proteomics, we identified more than 80 protein components from Lactobacillus-derived MVs, including some that were enriched in the vesicles themselves. For each species, vesicular proteins were categorized based on biological pathway and examined for subcellular localization signals in an effort to identify possible sorting mechanisms for MV proteins. Additionally, differences between MVs of other Lactobacillus species and Gram positive bacteria were highlighted. Information in this study will assist in elucidation of the formation and functions of MVs, as well as the development of therapeutic tools for vaccines, diagnosis, and therapeutic delivery.
HostingRepository	PRIDE
AnnounceDate	2019-11-12
AnnouncementXML	Submission_2019-11-12_15:23:32.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011278
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Scott Dean
SpeciesList	scientific name: Lactobacillus acidophilus NCFM; NCBI TaxID: 272621; scientific name: Lactobacillus casei subsp. casei ATCC 393; NCBI TaxID: 219334; scientific name: Lactobacillus reuteri DSM 20016; NCBI TaxID: 557436;
ModificationList	Deamidated; Oxidation
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2018-10-04 03:10:01	ID requested
⏵ 1	2019-11-12 15:23:33	announced
2	2024-10-22 04:49:31	announced	2024-10-22: Updated project metadata.

Dean SN, Leary DH, Sullivan CJ, Oh E, Walper SA, Isolation and characterization of Lactobacillus-derived membrane vesicles. Sci Rep, 9(1):877(2019) [pubmed]

curator keyword: Biological

submitter keyword: Lactobacillus, vesicles

Scott A. Walper
contact affiliation	Center for Bio/Molecular Science & Engineering (Code 6900), US Naval Research Laboratory, Washington DC, USA
contact email	scott.walper@nrl.navy.mil
lab head
Scott Dean
contact affiliation	Naval Research Laboratory
contact email	scottnathandean@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD011278

PRIDE project URI

• PRIDE
  1. PXD011278
     1. Label: PRIDE project
     2. Name: Lactobacillus Membrane Vesicles