PXD014871 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic changes in human sperm during sequential in vitro capacitation and acrosome reaction. |
Description | The male gamete is not completely mature after ejaculation and requires further events in the female genital tract to acquire fertilizing ability, including the processes of capacitation and acrosome reaction. In order to shed light on dynamic protein changes experienced by the sperm cell in preparation for fertilization, a comprehensive quantitative proteomic profiling based on isotopic peptide labeling and liquid chromatography followed by tandem mass spectrometry was performed on spermatozoa from three donors of proven fertility under three sequential conditions: purification with density gradient centrifugation, incubation with capacitation medium, and induction of acrosome reaction by the exposure to the calcium ionophore A23187. After applying strict selection criteria for peptide quantification and for statistical analyses, 36 proteins with significant changes in their relative abundance within sperm protein extracts were detected. Moreover, the presence of peptide residues potentially harboring sites for post-translational modification was revealed, suggesting that protein modification may be an important mechanism in sperm maturation. In this regard, increased levels of proteins mainly involved in motility and signaling, both regulated by protein modifiers, were detected in sperm lysates following incubation with capacitation medium. In contrast, less abundant proteins in acrosome-reacted cell lysates did not contain potentially modifiable residues, suggesting the possibility that all those proteins might be relocated or released during the process. Protein-protein interaction analysis revealed a subset of proteins potentially involved in sperm maturation, including ERLIN2, GGH, TMED 10, and ATP synthases. These results contribute to the current knowledge of the molecular basis of human fertilization and propose new candidates to be validated as modulators of male fertility. |
HostingRepository | PRIDE |
AnnounceDate | 2019-12-18 |
AnnouncementXML | Submission_2019-12-18_00:30:27.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD014871 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Judit Castillo |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | residues isobaric at 113.084064 Da; TMT6plex; Oxidation; Carbamidomethyl |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-08-01 03:29:17 | ID requested | |
1 | 2019-11-28 02:19:14 | announced | |
⏵ 2 | 2019-12-18 00:30:29 | announced | 2019-12-18: Updated project metadata. |
3 | 2020-01-14 07:23:27 | announced | 2019-12-18: Updated project metadata.
2020-01-14: Updated publication reference for PubMed record(s): 31824947. |
Publication List
Castillo J, Bogle OA, Jodar M, Torabi F, Delgado-Due, ñ, as D, Estanyol JM, Ballesc, à JL, Miller D, Oliva R, Capacitation and Acrosome Reaction. Front Cell Dev Biol, 7():295(2019) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: sperm, spermatozoa, capacitation, acrosome reaction, proteomics, mass spectrometry, TMT labeling |
Contact List
Rafael Oliva Virgili |
contact affiliation | Molecular Biology of Reproduction and Development Research Group; Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS); Fundació Clínic per a la Recerca Biomèdica (FCRB); Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universitat de Barcelona; Biochemistry and Molecular Genetics Service, Hospital Clínic, Barcelona, Spain |
contact email | roliva@ub.edu |
lab head | |
Judit Castillo |
contact affiliation | Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), University of Barcelona |
contact email | juditcastillo@ub.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD014871 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014871
- Label: PRIDE project
- Name: Proteomic changes in human sperm during sequential in vitro capacitation and acrosome reaction.